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不同提取方法对波罗蜜(Artocarpus heterophyllus L.)J33品种果实废弃物抗氧化潜力的评估及通过液相色谱-质谱联用仪鉴定酚类成分

Evaluation of antioxidant potential of Artocarpus heterophyllus L. J33 variety fruit waste from different extraction methods and identification of phenolic constituents by LCMS.

作者信息

Daud Mohd Nazrul Hisham, Fatanah Dian Nashiela, Abdullah Noriham, Ahmad Rohaya

机构信息

Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia; Food Safety and Forensic Department, Malaysian Agricultural Research and Development Institute, 43400 Serdang, Selangor, Malaysia.

Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.

出版信息

Food Chem. 2017 Oct 1;232:621-632. doi: 10.1016/j.foodchem.2017.04.018. Epub 2017 Apr 5.

Abstract

Artocarpus heterophyllus J33 (AhJ33) fruit is a popular and valuable jackfruit variety in Malaysia. For export, the pulp has to be separated from the skin which is usually discarded. Hence, the conversion of the fruit waste to food products with economic value needs to be explored utilizing the waste to wealth concept. This paper reports the evaluation of antioxidant potential of AhJ33 fruit waste (rind and rachis) extracts from three different extraction methods (maceration, percolation and Soxhlet). The antioxidant potential was assessed by DPPH radical scavenging, FRAP and β-carotene bleaching assays. The total phenolic and total flavonoid contents were estimated by TPC and the TFC assays. For both rind and rachis, the maceration technique yielded extracts with the strongest antioxidant activities which correlated with the highest TPC and TFC values. TOF LCMS analyses identified two phenolic acids as the major constituents responsible for the antioxidant activity of the active extracts.

摘要

面包树J33(AhJ33)果实是马来西亚一种受欢迎且有价值的菠萝蜜品种。用于出口时,果肉必须与通常被丢弃的果皮分离。因此,需要利用变废为宝的理念探索将水果废料转化为具有经济价值的食品。本文报道了采用三种不同提取方法(浸渍法、渗漉法和索氏提取法)对AhJ33水果废料(果皮和果轴)提取物的抗氧化潜力进行的评估。通过DPPH自由基清除、FRAP和β-胡萝卜素漂白试验评估抗氧化潜力。通过TPC和TFC试验估算总酚和总黄酮含量。对于果皮和果轴,浸渍技术得到的提取物具有最强的抗氧化活性,这与最高的TPC和TFC值相关。TOF LCMS分析确定了两种酚酸是活性提取物抗氧化活性的主要成分。

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