Kalemci Orhan, Aydin Hasan Emre, Kizmazoglu Ceren, Kaya Ismail, Yılmaz Hulya, Arda Nuri M
Department of Neurosurgery, School of Medicine and Hospital, Dokuz Eylul University, Izmir, Turkey.
Department of Pharmacology, Eskisehir Osmangazi University, Eskisehir, Turkey.
J Korean Neurosurg Soc. 2017 May;60(3):355-361. doi: 10.3340/jkns.2016.0505.015. Epub 2017 May 1.
The aim of this study to investigate the normal values of erythropoietin (EPO) and neuroprotective effects of quercetin and mannitol on EPO and hematocrit levels after acute severe traumatic brain injury (TBI) in rat model.
A weight-drop impact acceleration model of TBI was used on 40 male Wistar rats. The animals were divided into sham (group I), TBI (group II), TBI+quercetin (50 mg/kg intravenously) (group III), and TBI+mannitol (1 mg/kg intravenously) (group IV) groups. The malondialdehyde, glutathione peroxidase, catalase, EPO, and hematocrit levels were measured 1 and 4 hour after injury. Two-way repeated measures analysis of variance and Tukey's test were used for statistical analysis.
The malondialdehyde levels decreased significantly after administration of quercetin and mannitol compared with those in group II. Catalase and glutathione peroxidase levels increased significantly in groups III and IV. Serum EPO levels decreased significantly after mannitol but not after quercetin administration. Serum hematocrit levels did not change significantly after quercetin and mannitol administration 1 hour after trauma. However, mannitol administration decreased serum hematocrit levels significantly after 4 hour.
This study suggests that quercetin may be a good alternative treatment for TBI, as it did not decrease the EPO levels.
本研究旨在探讨促红细胞生成素（EPO）的正常值，以及槲皮素和甘露醇对大鼠急性重度创伤性脑损伤（TBI）后EPO和血细胞比容水平的神经保护作用。
对40只雄性Wistar大鼠采用重物坠落撞击加速模型制作TBI。动物被分为假手术组（I组）、TBI组（II组）、TBI+槲皮素（静脉注射50mg/kg）组（III组）和TBI+甘露醇（静脉注射1mg/kg）组（IV组）。在损伤后1小时和4小时测量丙二醛、谷胱甘肽过氧化物酶、过氧化氢酶、EPO和血细胞比容水平。采用双向重复测量方差分析和Tukey检验进行统计分析。
与II组相比，给予槲皮素和甘露醇后丙二醛水平显著降低。III组和IV组的过氧化氢酶和谷胱甘肽过氧化物酶水平显著升高。甘露醇给药后血清EPO水平显著降低，但槲皮素给药后未降低。创伤后1小时给予槲皮素和甘露醇后血清血细胞比容水平无显著变化。然而，4小时后给予甘露醇显著降低了血清血细胞比容水平。
本研究表明，槲皮素可能是TBI的一种良好替代治疗方法，因为它不会降低EPO水平。
