两种胸苷激酶缺陷型单纯疱疹病毒表现出意想不到的毒力特性。

Two thymidine kinase deficient herpes simplex viruses exhibit unexpected virulence properties.

作者信息

Izumi K M, Stevens J G

机构信息

Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90024.

出版信息

Microb Pathog. 1988 Feb;4(2):145-53. doi: 10.1016/0882-4010(88)90056-3.

DOI:10.1016/0882-4010(88)90056-3

PMID:2849029

Abstract

Herpes simplex virus type 1 (HSV-1) strains ANG and ANG path expressed very low levels of thymidine kinase activity, and their replication in vitro was not significantly inhibited by arabinofuranosyl-thymine. However, after intracranial inoculation of mice, ANG and ANG path demonstrated median lethal doses and brain replication profiles that resembled the fully neurovirulent thymidine kinase positive strain 17 syn+. In short, ANG and ANG path demonstrated the neurovirulence characteristics of standard HSV strains that express high levels of thymidine kinase activity. In an extension of the pathogenesis studies, ANG path resembled 17 syn+ and killed mice after rear footpad inoculation. ANG, by contrast, did not kill after rear footpad inoculation. Thus, ANG path demonstrated neuroinvasiveness while ANG was apparently non-neuroinvasive. The significance of these results as they pertain to genes involved in pathogenesis is discussed.

摘要

1型单纯疱疹病毒（HSV-1）毒株ANG和ANG path的胸苷激酶活性表达水平极低，它们在体外的复制未被阿糖呋喃基胸腺嘧啶显著抑制。然而，在小鼠颅内接种后，ANG和ANG path表现出的半数致死剂量和脑内复制情况类似于完全具有神经毒性的胸苷激酶阳性毒株17 syn+。简而言之，ANG和ANG path表现出了表达高水平胸苷激酶活性的标准HSV毒株的神经毒性特征。在发病机制研究的拓展中，ANG path类似于17 syn+，在后足垫接种后可致小鼠死亡。相比之下，ANG在后足垫接种后不会导致小鼠死亡。因此，ANG path表现出神经侵袭性，而ANG显然无神经侵袭性。讨论了这些结果与发病机制相关基因的意义。