Fu Chaoping, Zhou Haipeng, Wang Yanan, Liu Dong, Li Junmeng, Deng Haijun, Qi Xiaolong, Chen Tao, Zhang Li-Ming, Li Guoxin
PCFM Lab and GDHPPC Lab, School of Materials Science and Engineering, Sun Yat-sen University.
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
Int J Nanomedicine. 2017 Apr 27;12:3365-3374. doi: 10.2147/IJN.S130322. eCollection 2017.
The intraoperative precision cleaning of lymph nodes (LNs) is an essential component of treating neoplastic disease. To develop efficient probes for the targeted detection of LNs that could act as carriers for the specific diagnosis and treatment of metastatic LNs in the future, dextran-coated iron oxide nanoclusters (DIONs) were synthesized using a one-pot coprecipitation procedure. These modified DIONs have good water dispersibility, cytocompatibility, an optimum size, and a stable, dark brown color for LN imaging. In this study, cytotoxicity was evaluated using lymphatic endothelial cells (LECs) to predict biosafety and biocompatibility. Most importantly, the effectiveness of DIONs in mapping perigastric LNs in Sprague Dawley rats following injection into the gastric submucosal layer was demonstrated. In addition, a long-term tracing in vivo (from 4 days to 3 months) indicated that the DIONs had good biosafety and biocompatibility according to an evaluation of the behavior and blood biochemistry of the rat and a histopathological examination of the important organs.
术中对淋巴结（LN）进行精准清扫是治疗肿瘤疾病的重要环节。为了开发能够靶向检测淋巴结的高效探针，以便未来可作为转移性淋巴结特异性诊断和治疗的载体，采用一锅共沉淀法合成了葡聚糖包被的氧化铁纳米簇（DION）。这些经过修饰的DION具有良好的水分散性、细胞相容性、最佳尺寸以及用于淋巴结成像的稳定深棕色。在本研究中，使用淋巴管内皮细胞（LEC）评估细胞毒性，以预测生物安全性和生物相容性。最重要的是，证明了将DION注射到Sprague Dawley大鼠胃黏膜下层后，其在绘制胃周淋巴结方面的有效性。此外，一项长达3个月（从4天到3个月）的体内长期追踪研究表明，根据对大鼠行为、血液生化指标的评估以及对重要器官的组织病理学检查，DION具有良好的生物安全性和生物相容性。
