Al-Turki Turki A, Baskin Carol C
National Center of Agricultural Technology, Life Science & Environmental, King Abdulaziz City for Science and Technology (KACST), Box 6068, Riyadh 11442, Saudi Arabia.
Department of Biology, University of Kentucky, Lexington, KY 40506, USA.
Saudi J Biol Sci. 2017 May;24(4):822-829. doi: 10.1016/j.sjbs.2016.06.009. Epub 2016 Jul 5.
Our purpose was to evaluate the usefulness of the germination vs. the X-ray test in determining the initial viability of seeds of eight wild species (, , , , , , and ) from Saudi Arabia. Several days were required to determine viability of all eight species via germination tests, while immediate results on filled/viable seeds were obtained with the X-ray test. Seeds of all the species, except , showed high viability in both the germination (98-70% at 25/15 °C, 93-66% at 35/25 °C) and X-ray (100-75%) test. Furthermore, there was general agreement between the germination (10% at 25/15 °C and 8% at 35/25 °C) and X-ray (5%) tests that seed viability of was very low, and X-ray analysis revealed that this was due to poor embryo development. Seeds of have physical dormancy, which was broken by scarification in concentrated sulfuric acid (10 min), and they exhibited high viability in both the germination (98% at 25/15 °C and 93% at 35/25 °C) and X-ray (98%) test. Most of the nongerminated seeds of the eight species except those of were alive as judged by the tetrazolium test (TZ). Thus, for the eight species examined, the X-ray test was a good and rapid predictor of seed viability.
我们的目的是评估发芽试验与X射线检测在确定来自沙特阿拉伯的8种野生植物种子（、、、、、、和）初始活力方面的有效性。通过发芽试验确定所有8个物种的活力需要数天时间，而X射线检测能立即得出饱满/有活力种子的结果。除了之外，所有物种的种子在发芽试验（25/15°C时为98 - 70%，35/25°C时为93 - 66%）和X射线检测（100 - 75%）中均显示出高活力。此外，发芽试验（25/15°C时为10%，35/25°C时为8%）和X射线检测（5%）在种子活力非常低这一点上达成了普遍共识，X射线分析表明这是由于胚发育不良所致。的种子具有物理休眠特性，通过在浓硫酸中划破种皮（10分钟）可打破休眠，并且它们在发芽试验（25/15°C时为98%，35/25°C时为93%）和X射线检测（98%）中均表现出高活力。根据四唑染色试验（TZ）判断，除了之外的8个物种中大多数未发芽的种子是有活力的。因此，对于所检测的8个物种而言，X射线检测是种子活力的一种良好且快速的预测方法。
