Al-Hammad B A, Al-Ammari B S
Biology Department, College of Science and Humanities Studies, Prince Sattam Bin Abdulaziz University, Al-Kharj, Box 292, Riyadh 11942, Saudi Arabia.
Saudi J Biol Sci. 2017 Sep;24(6):1424-1429. doi: 10.1016/j.sjbs.2017.04.004. Epub 2017 Apr 12.
Our objective was to evaluate the usefulness of the germination vs. the X-ray test in determining the initial viability of seeds of five wild species (, , , and ) from Saudi Arabia. Usually several days were required to determine the viability of all five species via germination tests. However, X-ray test will give immediate results on filled/viable seeds. Seeds of all species, except and showed high viability in both germination (96-72% at 25/15 °C, 94-70% at 35/25 °C) and X-ray (100-80%) test. Furthermore, there was a general agreement between the germination (19%, 14% at 25/15 °C and 17% and 12% at 35/25 °C) and X-ray (8%, 4%) tests in which seed viability of and was very low due to insect damaged embryo as shown in X-ray analysis. Seeds of have physical dormancy, which was broken by scarification in concentrated sulfuric acid (10 min), and they exhibited high viability in both the germination (83% at 25/15 °C and 81% at 35/25 °C) and X-ray (96%) tests. Most of the nongerminated seeds of the five species except those of and , were alive as judged by the tetrazolium test (TZ). Thus, for the five species examined, the X-ray test was proved to be a good and rapid predictor of seed viability.
我们的目标是评估发芽试验与X射线检测在确定来自沙特阿拉伯的五个野生物种（、、、和）种子初始活力方面的有用性。通常需要几天时间才能通过发芽试验确定所有五个物种的活力。然而，X射线检测能立即给出饱满/有活力种子的结果。除了和之外，所有物种的种子在发芽试验（25/15°C时为96 - 72%，35/25°C时为94 - 70%）和X射线检测（100 - 80%）中均显示出高活力。此外，发芽试验（25/15°C时为19%、14%，35/25°C时为17%、12%）和X射线检测（8%、4%）结果总体一致，其中和的种子活力非常低，因为X射线分析显示其胚受到昆虫损害。的种子具有物理休眠特性，通过在浓硫酸中划痕（10分钟）打破休眠，它们在发芽试验（25/15°C时为83%，35/25°C时为81%）和X射线检测（96%）中均表现出高活力。除了和的种子外，通过四唑盐试验（TZ）判断，五个物种的大多数未发芽种子都是有活力的。因此，对于所检测的五个物种，X射线检测被证明是种子活力的良好且快速的预测方法。
