Dos Santos Francine Melise, Piffer Alícia Corbellini, Schneider Rafael de Oliveira, Ribeiro Nicole Sartori, Garcia Ane Wichine Acosta, Schrank Augusto, Kmetzsch Lívia, Vainstein Marilene Henning, Staats Charley Christian
Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
Future Microbiol. 2017 May;12:491-504. doi: 10.2217/fmb-2016-0160. Epub 2017 May 11.
To evaluate alterations of zinc homeostasis in macrophages exposed to Cryptococcus neoformans. Materials & methods: Using a fluorescent zinc probe-based flow cytometry and atomic absorption spectrometry, zinc levels were evaluated in J774.A1 cell lines exposed to C. neoformans H99 cells. The transcription profile of macrophage zinc related homeostasis genes - metallothioneins and zinc transporters (ZnTs) of the SLC30 and SLC39 (Zrt-Irt-protein) families - was analyzed by quantitative PCR.
Macrophage intracellular labile zinc levels decreased following exposure to C. neoformans. A significant decrease in transcription levels was detected in specific ZnTs from both the Zrt-Irt-protein and ZnT families, especially 24 h after infection.
These findings suggest that macrophages may exhibit zinc depletion in response to C. neoformans infection.
评估暴露于新型隐球菌的巨噬细胞中锌稳态的变化。材料与方法：使用基于荧光锌探针的流式细胞术和原子吸收光谱法，评估暴露于新型隐球菌H99细胞的J774.A1细胞系中的锌水平。通过定量PCR分析巨噬细胞锌相关稳态基因——SLC30和SLC39（Zrt-Irt-蛋白）家族的金属硫蛋白和锌转运体（ZnTs）的转录谱。
暴露于新型隐球菌后，巨噬细胞内不稳定锌水平降低。在Zrt-Irt-蛋白家族和ZnT家族的特定ZnTs中检测到转录水平显著下降，尤其是在感染后24小时。
这些发现表明，巨噬细胞可能会因新型隐球菌感染而出现锌缺乏。
