鼠巨噬细胞系中新型隐球菌对锌稳态的影响。

Alterations of zinc homeostasis in response to Cryptococcus neoformans in a murine macrophage cell line.

作者信息

Dos Santos Francine Melise, Piffer Alícia Corbellini, Schneider Rafael de Oliveira, Ribeiro Nicole Sartori, Garcia Ane Wichine Acosta, Schrank Augusto, Kmetzsch Lívia, Vainstein Marilene Henning, Staats Charley Christian

机构信息

Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.

Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.

出版信息

Future Microbiol. 2017 May;12:491-504. doi: 10.2217/fmb-2016-0160. Epub 2017 May 11.

DOI:10.2217/fmb-2016-0160

PMID:28492340

Abstract

AIM

To evaluate alterations of zinc homeostasis in macrophages exposed to Cryptococcus neoformans. Materials & methods: Using a fluorescent zinc probe-based flow cytometry and atomic absorption spectrometry, zinc levels were evaluated in J774.A1 cell lines exposed to C. neoformans H99 cells. The transcription profile of macrophage zinc related homeostasis genes - metallothioneins and zinc transporters (ZnTs) of the SLC30 and SLC39 (Zrt-Irt-protein) families - was analyzed by quantitative PCR.

RESULTS

Macrophage intracellular labile zinc levels decreased following exposure to C. neoformans. A significant decrease in transcription levels was detected in specific ZnTs from both the Zrt-Irt-protein and ZnT families, especially 24 h after infection.

CONCLUSION

These findings suggest that macrophages may exhibit zinc depletion in response to C. neoformans infection.

摘要

目的

评估暴露于新型隐球菌的巨噬细胞中锌稳态的变化。材料与方法：使用基于荧光锌探针的流式细胞术和原子吸收光谱法，评估暴露于新型隐球菌H99细胞的J774.A1细胞系中的锌水平。通过定量PCR分析巨噬细胞锌相关稳态基因——SLC30和SLC39（Zrt-Irt-蛋白）家族的金属硫蛋白和锌转运体（ZnTs）的转录谱。