Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Lab Chip. 2017 Jun 13;17(12):2067-2075. doi: 10.1039/c6lc01568a.
Biocompatible microparticles are valuable tools in biomedical research for applications such as drug delivery, cell transplantation therapy, and analytical assays. However, their translation into clinical research and the pharmaceutical industry has been slow due to the lack of techniques that can produce microparticles with controlled physicochemical properties at high throughput. We introduce a robust microfluidic platform for the production of relatively homogeneous microdroplets at a generation frequency of up to 3.1 MHz, which is about three orders of magnitude higher than the production rate of a conventional microfluidic drop maker. We demonstrated the successful implementation of our device for production of biocompatible microparticles with various crosslinking mechanisms and cell microencapsulation with high cell viability.
生物相容性微球在生物医药研究中是一种很有价值的工具，可应用于药物输送、细胞移植治疗和分析检测等领域。然而，由于缺乏能够高通量制备具有可控物理化学性质的微球的技术，其向临床研究和制药行业的转化一直较为缓慢。我们介绍了一种强大的微流控平台，该平台能够以高达 3.1 MHz 的生成频率产生相对均匀的微液滴，这比传统微流控滴头的生成速率高出约三个数量级。我们成功地展示了我们的设备在制备具有各种交联机制的生物相容性微球和高细胞活力的细胞微囊化方面的应用。
