Dahodwala Hussain, Sharfstein Susan T
Vaccine production program (VPP), VRC/NIAID/NIH, Gaithersburg, MD, 20878, USA.
SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY, 12203, USA.
Methods Mol Biol. 2017;1603:153-168. doi: 10.1007/978-1-4939-6972-2_10.
Increased understanding of Chinese hamster ovary (CHO) cell physiology has been ushered in upon availability of the parental CHO-K1 cell line genome. Free and openly accessible sequence information has complemented transcriptomic and proteomic studies. The previous decade has also seen an increase in sensitivity and accuracy of proteomic methods due to technology development. In this genomic era, high-throughput screening methods, sophisticated informatic tools, and models continually drive major innovations in cell line development and process engineering. This review describes the various achievements in 'omics techniques and their application to improve recombinant protein expression from CHO cell lines.
随着亲本CHO-K1细胞系基因组的可得，人们对中国仓鼠卵巢（CHO）细胞生理学有了更多的了解。免费且可公开获取的序列信息补充了转录组学和蛋白质组学研究。由于技术发展，过去十年蛋白质组学方法的灵敏度和准确性也有所提高。在这个基因组时代，高通量筛选方法、精密的信息工具和模型不断推动细胞系开发和工艺工程的重大创新。本综述描述了“组学”技术的各项成就及其在提高CHO细胞系重组蛋白表达方面的应用。
