Suwanwela Jaijam, Puangchaipruk Dollaya, Wattanasirmkit Kamolporn, Kamolratanakul Paksinee, Jansisyanont Pornchai
Int J Oral Maxillofac Implants. 2017 May/Jun;32(3):611-616. doi: 10.11607/jomi.5052.
Many histologic and histomorphometric studies as well as systematic reviews have shown the clinical success of the use of anorganic bovine bone (ABB, Bio-Oss) in maxillary sinus floor augmentation (MSFA). The molecular processes involved in bone healing are, however, still unknown. The aims of this study were to explore gene expression associated with bone remodeling and inflammation in MSFA sites.
The mRNA expression levels of runt related transcription factor 2 (RUNX2), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metallopeptidase 9 (MMP-9), tartrate-resistance acid phosphatase (TRAP), and interleukin-1beta (IL-1β), as well as the ratio of RANKL/OPG were compared between alveolar bone of a group after MSFA with ABB and a maxillary posterior edentulous bone group. Twenty-one bone samples were collected at the time of implant placement after 6 months of MSFA or tooth extraction. Fourteen bone samples from the MSFA group and from the maxillary posterior edentulous bone without MSFA group were taken to analyze gene expression by real-time reverse transcription polymerase chain reaction (RT-PCR). Seven bone samples from the MSFA group were used for histologic analysis.
Real time RT-PCR revealed no statistically significant difference in gene expression level of RUNX2, RANKL, OPG, MMP-9, TRAP, and IL-1β, or in the ratio of RANKL/OPG. Histology showed bone-lining cells at the edge and osteocyte inside newly formed bone. Residual grafted particles were in close contact with new bone.
After a healing period of 6 months, ABB particles did not have an effect on the expression of genes associated with bone remodeling and inflammation. In addition, histologic evidence supports that ABB particles are replaced by new bone formation and do not affect bone healing.
许多组织学和组织形态计量学研究以及系统评价均已表明，使用无机牛骨（ABB，Bio - Oss）进行上颌窦底提升术（MSFA）具有临床成功性。然而，骨愈合过程中涉及的分子机制仍不清楚。本研究的目的是探索与MSFA部位骨重塑和炎症相关的基因表达情况。
比较了使用ABB进行MSFA后的一组牙槽骨与上颌后牙区无牙颌骨组之间，与 runt 相关转录因子 2（RUNX2）、核因子κB 受体活化因子配体（RANKL）、骨保护素（OPG）、基质金属蛋白酶 9（MMP - 9）、抗酒石酸酸性磷酸酶（TRAP）和白细胞介素 - 1β（IL - 1β）的 mRNA 表达水平，以及 RANKL/OPG 比值。在MSFA或拔牙6个月后的种植体植入时收集了21份骨样本。从MSFA组和未进行MSFA的上颌后牙区无牙颌骨组中分别采集14份骨样本，通过实时逆转录聚合酶链反应（RT - PCR）分析基因表达。从MSFA组中选取7份骨样本进行组织学分析。
实时RT - PCR显示，RUNX2、RANKL、OPG、MMP - 9、TRAP和IL - 1β的基因表达水平或RANKL/OPG比值在统计学上无显著差异。组织学显示，新形成骨边缘有骨衬细胞，内部有骨细胞。残留的移植颗粒与新骨紧密接触。
经过6个月的愈合期后，ABB颗粒对与骨重塑和炎症相关的基因表达没有影响。此外，组织学证据支持ABB颗粒被新骨形成所替代，且不影响骨愈合。
