Yan Qiuting, Fang Lipao, Wei Jiyu, Xiao Guipeng, Lv Meihong, Ma Quanhong, Liu Chunfeng, Wang Wang
a College of Pharmaceutical Sciences , Soochow University , Suzhou , China.
b Institute of Neuroscience , Soochow University , Suzhou , China.
J Biomater Sci Polym Ed. 2017 Sep;28(13):1394-1407. doi: 10.1080/09205063.2017.1329888. Epub 2017 May 17.
Owing to its biocompatibility, noncytotoxicity, biodegradability and three-dimensional structure, vertically silicon nanowires (SiNWs) arrays are a promising scaffold material for tissue engineering, regenerative medicine and relevant medical applications. Recently, its osteogenic differentiation effects, reorganization of cytoskeleton and regulation of the fate on stem cells have been demonstrated. However, it still remains unknown whether SiNWs arrays could affect the proliferation and neuronal differentiation of neural stem cells (NSCs) or not. In the present study, we have employed vertically aligned SiNWs arrays as culture systems for NSCs and proved that the scaffold material could promote the proliferation and neuronal differentiation of NSCs while maintaining excellent cell viability and stemness. Immunofluorescence imaging analysis, Western blot and RT-PCR results reveal that NSCs proliferation and neuronal differentiation efficiency on SiNWs arrays are significant greater than that on silicon wafers. These results implicate SiNWs arrays could offer a powerful platform for NSCs research and NSCs-based therapy in the field of neural tissue engineering.
由于其生物相容性、无细胞毒性、生物可降解性和三维结构，垂直硅纳米线（SiNWs）阵列是一种在组织工程、再生医学及相关医学应用中很有前景的支架材料。最近，已经证实了其对干细胞的成骨分化作用、细胞骨架重组和命运调控。然而，SiNWs阵列是否会影响神经干细胞（NSCs）的增殖和神经元分化仍不清楚。在本研究中，我们将垂直排列的SiNWs阵列用作NSCs的培养系统，并证明该支架材料可以促进NSCs的增殖和神经元分化，同时保持优异的细胞活力和干性。免疫荧光成像分析、蛋白质印迹和逆转录聚合酶链反应结果表明，NSCs在SiNWs阵列上的增殖和神经元分化效率显著高于在硅片上的效率。这些结果表明，SiNWs阵列可为神经组织工程领域的NSCs研究和基于NSCs的治疗提供一个强大的平台。
