Wu Chao, Xue Lan-De, Su Lin-Wang, Xie Jian-Li, Jiang Huan, Yu Xi-Jiao, Liu Hong-Mei
a Department of Neurosurgery , Tengzhou Central People's Hospital , Shandong , China.
b Department of Periodontology , Jinan Stomatological Hospital , Jinan , China.
Neurol Res. 2019 Mar;41(3):208-215. doi: 10.1080/01616412.2018.1544400. Epub 2018 Dec 30.
Neural stem cells (NSCs) are multipotent stem cells that generating various neural cells, including neurons, astrocytes and oligodendrocytes. This showed that NSCs is an ideal candidate in the application of neural disease treatment. In the current study, we established a simple and efficient method to promote the viability and induce the differentiation of NSCs by stimulating with magnesium.
The proliferation and differentiation of NSCs was determined by MTT assay and immunostaining. The behavior alteration was measured by rotorod test and Morris water maze.
Magnesium enhanced proliferation in NSCs. The ratio of Nestin+, Ki67+ and GFAP+ progenitor cells was increased in the presence of magnesium. Besides, magnesium induced the glial differentiation instead of neuronal differentiation in NSCs. By contrast, transplantation of Mg2+-treated NSCs in vivo generated more neurons. In established PD models, transplantation of Mg2+-treated NSCs could improve the symptoms and recover the memory.
We established a simple and efficient way to promote the proliferation and induce the differentiation of NSCs. More importantly, this may also facilitate to develop a new method to neural disorder treatment.
神经干细胞（NSCs）是多能干细胞，可生成各种神经细胞，包括神经元、星形胶质细胞和少突胶质细胞。这表明神经干细胞是神经疾病治疗应用中的理想候选者。在本研究中，我们建立了一种简单有效的方法，通过镁刺激来提高神经干细胞的活力并诱导其分化。
通过MTT法和免疫染色测定神经干细胞的增殖和分化。通过转棒试验和莫里斯水迷宫测量行为改变。
镁增强了神经干细胞的增殖。在镁存在的情况下，巢蛋白阳性、Ki67阳性和胶质纤维酸性蛋白阳性祖细胞的比例增加。此外，镁在神经干细胞中诱导胶质细胞分化而非神经元分化。相比之下，体内移植经镁处理的神经干细胞可产生更多神经元。在已建立的帕金森病模型中，移植经镁处理的神经干细胞可改善症状并恢复记忆。
我们建立了一种简单有效的方法来促进神经干细胞的增殖并诱导其分化。更重要的是，这也可能有助于开发一种治疗神经疾病的新方法。
