人类多瘤病毒JC病毒增强子含有核因子I结合序列；使用小鼠脑海核提取物进行分析。
Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts.
作者信息
Tamura T, Inoue T, Nagata K, Mikoshiba K
机构信息
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
出版信息
Biochem Biophys Res Commun. 1988 Dec 15;157(2):419-25. doi: 10.1016/s0006-291x(88)80265-1.
Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations (Khalili, K., Rappaport, J. and Khoury, G. (1988) EMBO J. 7, 1205-1210 (20], the NFI motif is suggested to play an important role in early gene expression of JCV.
神经营养性人JC病毒携带一个负责组织特异性基因表达的98碱基对重复增强子。使用小鼠脑细胞核提取物进行的DNase I足迹分析研究分别在增强子上游（229位）和中间（156和58位）揭示了弱（假NFI基序）和强（NFI基序）核因子I结合序列。在脑提取物中驱动的体外转录表明，NFI基序是一个可能的转录控制元件。结合先前的观察结果（哈利利，K.，拉帕波特，J.和库里，G.（1988年）《欧洲分子生物学组织杂志》7，1205 - 1210 [20]），提示NFI基序在JCV的早期基因表达中起重要作用。
Zotero 插件