来自阿尔加维海岸的嗜极植物体外抗利什曼原虫特性的首次报告。
First report of the in vitro antileishmanial properties of extremophile plants from the Algarve Coast.
作者信息
Oliveira Marta, João Rodrigues Maria, Pereira Catarina, Neto Rubens Lima do Monte, Junior Policarpo Ademar Santos, Neng Nuno da Rosa, Nogueira José Manuel Florêncio, Varela João, Barreira Luísa, Custódio Luísa
机构信息
a Faculty of Sciences and Technology , Centre of Marine Sciences, University of Algarve , Faro , Portugal.
b Centro de Pesquisas René Rachou, FIOCRUZ , Belo Horizonte , Brazil.
出版信息
Nat Prod Res. 2018 Mar;32(5):600-604. doi: 10.1080/14786419.2017.1326489. Epub 2017 May 11.
This work reports for the first time the in vitro anti Leishmania infantum activity of acetone and dichloromethane (DCM) extracts from 25 extremophile plants from Southern Portugal. DCM extracts from Inula chritmoides and Spergularia rubra were active against axenic promastigotes and intracellular amastigotes, had anti-inflammatory properties on lipopolysaccharide (LPS)-stimulated macrophages, inhibited acetylcholinesterase and had no haemolytic activity on human erythrocytes. Eleven phenolics were identified by high-performance liquid chromatography with diode-array detection (HPLC-DAD) in I. crithmoides and one phenolic in S. rubra. Isolation and identification of the active molecules is in progress.
这项研究首次报告了葡萄牙南部25种极端植物的丙酮提取物和二氯甲烷（DCM）提取物对婴儿利什曼原虫的体外活性。来自菊苣旋覆花和小红花马齿苋的DCM提取物对无菌前鞭毛体和细胞内无鞭毛体具有活性，对脂多糖（LPS）刺激的巨噬细胞具有抗炎特性，抑制乙酰胆碱酯酶，并且对人红细胞无溶血活性。通过高效液相色谱-二极管阵列检测（HPLC-DAD）在菊苣旋覆花中鉴定出11种酚类物质，在小红花马齿苋中鉴定出1种酚类物质。活性分子的分离和鉴定正在进行中。
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