Ben Brahim Raoua, Ellouzi Hasna, Fouzai Khaoula, Asses Nedra, Neffati Mohammed, Sabatier Jean Marc, Bulet Philippe, Regaya Imed
Laboratory of Extremophile Plants, Centre of Biotechnology of Borj Cedria, B.P. 901, Hammam-Lif 2050, Tunisia.
Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia.
Antibiotics (Basel). 2022 Sep 24;11(10):1302. doi: 10.3390/antibiotics11101302.
Extraction methods depend mainly on the chemical nature of the extracted molecule. For these reasons, the selection of the extraction medium is a vital part of obtaining these molecules. The extraction of antimicrobial peptides (AMPs) from extremophile plants is important because of its potential pharmaceutical applications. This work focused on the evaluation of several solvents for the extraction of AMPs from the following two extremophile plants: and from southern Tunisia. In order to identify the most efficient solvents and extraction solutions, we used sulfuric acid, dichloromethane, phosphate buffer, acetic acid and sodium acetate, and we tested them on leaves and roots of both the studied plants. The extracts obtained using sulfuric acid, dichloromethane and phosphate buffer extraction did not show any antimicrobial activity, whereas the acetic acid and sodium acetate extracts led to growth inhibition of some of the tested bacterial strains. The extracts of leaves and roots of and obtained by acetic acid and sodium acetate were proven to be active against Gram-positive bacteria and Gram-negative bacteria. Therefore, the most appropriate solvents to use for antimicrobial peptide extraction from both plants are acetic acid and sodium acetate.
提取方法主要取决于被提取分子的化学性质。基于这些原因，提取介质的选择是获取这些分子的关键部分。从嗜极植物中提取抗菌肽（AMPs）因其潜在的药物应用而具有重要意义。这项工作着重评估了几种用于从突尼斯南部的以下两种嗜极植物中提取抗菌肽的溶剂：[此处原文缺失两种植物的具体名称]。为了确定最有效的溶剂和提取溶液，我们使用了硫酸、二氯甲烷、磷酸盐缓冲液、乙酸和乙酸钠，并在两种受试植物的叶子和根部进行了测试。使用硫酸、二氯甲烷和磷酸盐缓冲液提取得到的提取物未显示出任何抗菌活性，而乙酸和乙酸钠提取物对一些受试细菌菌株具有生长抑制作用。经证实，通过乙酸和乙酸钠从[此处原文缺失两种植物的具体名称]的叶子和根部获得的提取物对革兰氏阳性菌和革兰氏阴性菌均有活性。因此，从这两种植物中提取抗菌肽最适宜使用的溶剂是乙酸和乙酸钠。
