Oprandy J J, Schwan T G, Main A J
Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, CT 06510.
Can J Microbiol. 1988 Jun;34(6):782-6. doi: 10.1139/m88-133.
Five new isolates of Kemerovo group viruses were recovered from Ixodes uriae collected on Great Island, Witless Bay Seabird Sanctuary, Newfoundland, Canada, during July 1985. This brings the total number of Orbivirus isolates on Great Island to 18 isolates including the 7 from 1971 and 6 from 1972. Genomic segments of several strains were compared by polyacrylamide gel electrophoresis. The degree of variation in each segment of these viruses was calculated. Great Island and Bauline viruses exhibited a great degree of variation in dsRNA migration patterns. Great Island and Bauline genomes averaged 11.60 (SD = 0.107) and 11.69 megadaltons (SD = 0.075), respectively. Variation was observed in all 10 segments of Great Island and Bauline viruses. These findings were compared with serologic and protein gel data.
1985年7月，从加拿大纽芬兰威特利斯湾海鸟保护区大岛上采集的海鸟蜱中分离出5株新的克麦罗沃组病毒。这使得大岛上分离出的环状病毒总数达到18株，其中包括1971年的7株和1972年的6株。通过聚丙烯酰胺凝胶电泳对几株病毒的基因组片段进行了比较。计算了这些病毒各片段的变异程度。大岛病毒和鲍林病毒在双链RNA迁移模式上表现出很大程度的变异。大岛病毒和鲍林病毒基因组的平均分子量分别为11.6０（标准差 = 0.107）和11.69兆道尔顿（标准差 = 0.075）。在大岛病毒和鲍林病毒的所有10个片段中均观察到变异。将这些结果与血清学和蛋白质凝胶数据进行了比较。
