Chitimia-Dobler Lidia, Langguth Johanna, Pfeffer Martin, Kattner Simone, Küpper Thomas, Friese Daniela, Dobler Gerhard, Guglielmone Alberto A, Nava Santiago
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D80937 Munich, German Center of Infection Research (DZIF) Partner Munich, Germany.
Institute of Animal Hygiene and Veterinary Public Health, Veterinary Faculty, University of Leipzig, Germany.
Vet Parasitol. 2017 May 30;239:1-6. doi: 10.1016/j.vetpar.2017.04.012. Epub 2017 Apr 13.
The aim of this work was to determine the evolutionary relationship among tick populations of Rhipicephalus sanguineus sensu lato distributed in Africa north of the Sahara and different lineages of R. sanguineus s.l. distributed in different regions of Sub-Saharan Africa, America and Europe through the analysis of DNA sequences of two mitochondrial genes. One hundred and thirty six 16S rRNA gene sequences and twenty-seven 12S rRNA gene sequences of R. sanguineus s.l. were analyzed. Phylogenetic analyses were performed including different lineages of R. sanguineus s.l. from America, Europe and Africa, and species belonging to the R. sanguineus group as Rhipicephalus camicasi, Rhipicephalus guilhoni, Rhipicephalus sulcatus, Rhipicephalus rossicus, Rhipicephalus pusillus, Rhipicephalus turanicus and Rhipicephalus leporis. At least two different lineages of R. sanguineus s.l. are living in sympatry in Africa north of the Sahara. One of these mitochondrial lineages belongs to the same evolutionary entity that R. sanguineus s.l. from tropical areas of America, R. sanguineus s.l. from Sub-Saharan Africa, R. camicasi and R. guilhoni. The other mitochondrial lineage of R. sanguineus s.l. present in Africa north of the Sahara is phylogenetically associated to R. sanguineus s.l. ticks from southeastern Europe (Romania, Turkey and Greece). Both evolutionary entities are clearly different to the evolutionary entity formed by R. sanguineus s.l. from western Europe and temperate areas of America. Thus, the name R. sanguineus s.s. cannot be assigned to any of the two evolutionary entities present in Africa north of the Sahara. The taxonomic status of these taxa will remain unresolved until new lines of evidence become available to complement the current results based on mitochondrial DNA.
本研究的目的是通过分析两个线粒体基因的DNA序列，确定分布在撒哈拉以北非洲的狭义血红扇头蜱种群与分布在撒哈拉以南非洲、美洲和欧洲不同地区的狭义血红扇头蜱不同谱系之间的进化关系。分析了136条狭义血红扇头蜱的16S rRNA基因序列和27条12S rRNA基因序列。进行了系统发育分析，包括来自美洲、欧洲和非洲的狭义血红扇头蜱不同谱系，以及属于血红扇头蜱组的物种，如卡米卡西血红扇头蜱、吉洪尼血红扇头蜱、沟纹血红扇头蜱、罗斯西血红扇头蜱、微小血红扇头蜱、图兰血红扇头蜱和兔血红扇头蜱。在撒哈拉以北的非洲，至少有两个不同的狭义血红扇头蜱谱系共存。其中一个线粒体谱系属于与来自美洲热带地区的狭义血红扇头蜱、来自撒哈拉以南非洲的狭义血红扇头蜱、卡米卡西血红扇头蜱和吉洪尼血红扇头蜱相同的进化实体。在撒哈拉以北非洲存在的狭义血红扇头蜱的另一个线粒体谱系在系统发育上与来自东南欧（罗马尼亚、土耳其和希腊）的狭义血红扇头蜱相关。这两个进化实体与由来自西欧和美洲温带地区的狭义血红扇头蜱形成的进化实体明显不同。因此，不能将狭义血红扇头蜱这个名称赋予撒哈拉以北非洲存在的两个进化实体中的任何一个。在有新的证据补充基于线粒体DNA的当前结果之前，这些分类单元的分类地位仍无法确定。
