Arafat Nagah, Eladl Abdelfattah H, Mahgoub Hebatallah, El-Shafei Reham A
Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Vaccine. 2017 Jun 22;35(29):3682-3689. doi: 10.1016/j.vaccine.2017.04.076. Epub 2017 May 9.
Chickens infected with both infectious bursal disease virus (IBDV) and Salmonella had higher mortality. In this work, we investigated the effect of IBDV vaccine (modified live-virus bursal disease vaccine, Nobilis strain 228E®) on experimentally infected chickens with Salmonella Enteritidis (SE).
Four experimental groups were included in this study, negative control group, 228E®group, 228E®+SE infected group, and SE infected group. Chickens were ocularly administrated 228E® at 12days of age and orally infected with S. Enteritidis at 13days of age. Sera, intestinal fluid, blood, cloacal swabs and tissue samples were collected at 1, 2 and 3weeks post vaccination (PV).
The recorded mortalities were higher in the 228E®+SE infected group, compared to the SE infected group. The anti-S. Enteritidis serum antibody titer and the intestinal mucosal IgA level were higher in the SE infected group at 2 and 3weeks PV, compared to 228E®+SE infected group. S. Enteritidis fecal shedding and organ colonization were significantly higher in the 228E®+SE infected group than the SE infected group at 2 and 3weeks PV. The 228E®+SE group had significantly lower bursa to body weight ratios at 2 and 3weeks PV, as well as had higher bursal lesion scores than the SE infected group. IBDV vaccine depressed the specific-SE systemic and mucosal antibody responses, but did not affect the specific-SE cellular immune responses.
Chickens administrated IBDV vaccine, followed by S. Enteritidis infection, could cause a significant effect on the bursa of Fabricius, resulting in failure of systemic and mucosal antibody responses to the S. Enteritidis and reduce the elimination and the clearance of S. Enteritidis.
感染传染性法氏囊病病毒（IBDV）和沙门氏菌的鸡死亡率更高。在本研究中，我们调查了IBDV疫苗（改良活病毒法氏囊病疫苗，Nobilis株228E®）对实验性感染肠炎沙门氏菌（SE）的鸡的影响。
本研究包括四个实验组，阴性对照组、228E®组、228E®+SE感染组和SE感染组。鸡在12日龄时经眼接种228E®，并在13日龄时经口感染肠炎沙门氏菌。在接种疫苗后（PV）1、2和3周收集血清、肠液、血液、泄殖腔拭子和组织样本。
与SE感染组相比，228E®+SE感染组记录的死亡率更高。在PV 2周和3周时，SE感染组的抗肠炎沙门氏菌血清抗体滴度和肠黏膜IgA水平高于228E®+SE感染组。在PV 2周和3周时，228E®+SE感染组的肠炎沙门氏菌粪便排出量和器官定植显著高于SE感染组。在PV 2周和3周时，228E®+SE组的法氏囊与体重比显著低于SE感染组，且法氏囊病变评分高于SE感染组。IBDV疫苗抑制了特异性SE全身和黏膜抗体反应，但不影响特异性SE细胞免疫反应。
接种IBDV疫苗后再感染肠炎沙门氏菌的鸡，会对法氏囊产生显著影响，导致对肠炎沙门氏菌的全身和黏膜抗体反应失败，并减少肠炎沙门氏菌的清除和排出。
