Jumaa Rawaa Saladdin, Abdulmajeed Dhuha Ismael, Karim Abdulkarim Jafar
Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Vet World. 2021 Nov;14(11):2971-2978. doi: 10.14202/vetworld.2021.2971-2978. Epub 2021 Nov 25.
Infectious bursal disease attacks the poultry industry, mainly young chickens, causing immunosuppression, and death with high economic losses. This study aimed to evaluate the effects of the monoextract, diextracts, and triextracts of (QI), (CiA), and (CoA) on infectious bursal disease virus (IBDV) in embryonated chicken eggs (ECEs).
The experimental design consisted of three sets of ECEs at 11 days of age, and each set included seven groups (G1-G7). The extracts of QI, CiA, and CoA were inoculated to ECEs by the chorioallantoic membrane method before, in concomitant (mixed) with, and after IBDV infection to the first, second, and third sets, respectively. The monoextract, diextracts, and triextracts of QI, CiA, and CoA were given at 1%, 2%, 5%, and 10% concentrations to G1-G3, G4-G6, and G7, respectively. Real-time polymerase chain reaction identified and confirmed the virus in accordance with the pathological changes.
The monoextract (5-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas the monoextract (10% concentration) inhibited IBDV during mixed inoculation and post-inoculation. Diextracts (2-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas diextracts (5-10% concentrations) inhibited IBDV during mixed inoculation and post-inoculation. Triextracts (1%, 2%, 5%, and 10% concentrations) inhibited IBDV by ameliorating the pathological changes of the virus and preventing the death of ECEs.
The inoculation of herbal extracts, particularly triextracts, alleviates the pathological changes in ECEs infected with IBDV. This study recommends the oral route in evaluating plant extracts against IBDV in poultry.
传染性法氏囊病侵袭家禽业，主要感染幼鸡，导致免疫抑制和死亡，造成巨大经济损失。本研究旨在评估黄芪（QI）、肉桂（CiA）和辅酶A（CoA）的单提取物、双提取物和三提取物对鸡胚（ECEs）中传染性法氏囊病病毒（IBDV）的影响。
实验设计包括三组11日龄的鸡胚，每组包括七个组（G1 - G7）。分别在IBDV感染第一组、第二组和第三组鸡胚之前、同时（混合）和之后，通过绒毛尿囊膜法将QI、CiA和CoA的提取物接种到鸡胚中。QI、CiA和CoA的单提取物、双提取物和三提取物分别以1%、2%、5%和10%的浓度给予G1 - G3、G4 - G6和G7组。实时聚合酶链反应根据病理变化鉴定并确认病毒。
单提取物（浓度为5 - 10%）抑制IBDV，对病毒感染前接种无影响，而单提取物（浓度为10%）在混合接种和接种后抑制IBDV。双提取物（浓度为2 - 10%）抑制IBDV，对病毒感染前接种无影响，而双提取物（浓度为5 - 10%）在混合接种和接种后抑制IBDV。三提取物（浓度为1%、2%、5%和10%）通过改善病毒的病理变化和防止鸡胚死亡来抑制IBDV。
接种草药提取物，特别是三提取物，可减轻感染IBDV的鸡胚的病理变化。本研究建议采用口服途径评估植物提取物对家禽IBDV的作用。
