Wang Hong, Chen Jian, Hong Yongxiang, Lv Kun, Yu Maolin, Zhang Peisheng, Long Yunfei, Yi Pinggui
Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology.
Anal Sci. 2017;33(5):591-597. doi: 10.2116/analsci.33.591.
In this study, amphiphilic diblock copolymers were designed and synthesized via the incorporation of reversible addition-fragmentation chain transfer radical polymerization (RAFT) and a subsequent grafting technique. Subsequently, Hg-sensitive water-soluble fluorescent polymeric micelles (FNs) were prepared by a reprecipitation strategy. The spectroscopic characteristics demonstrate that the fluorescein isothiocyanate (FITC) was successfully linked into the polymer. Due to the promoted reaction of desulfurization cyclization by Hg, the fluorescence of fluorescein in FNs was obviously quenched. The as-prepared FNs showed admirable Hg-sensitivity (detection limit: 54 nM), excellent water-solubility and high selectivity. In addition, FNs were successfully used to determine Hg in blood serum. We expected that the as-prepared FNs could perform potential applications in imaging, sensing, and bioanalytic chemistry.
在本研究中，通过引入可逆加成-断裂链转移自由基聚合（RAFT）和后续接枝技术，设计并合成了两亲性二嵌段共聚物。随后，采用再沉淀策略制备了对汞敏感的水溶性荧光聚合物胶束（FNs）。光谱特征表明，异硫氰酸荧光素（FITC）成功连接到聚合物中。由于汞促进了脱硫环化反应，FNs中荧光素的荧光明显猝灭。所制备的FNs表现出令人满意的汞敏感性（检测限：54 nM）、优异的水溶性和高选择性。此外，FNs成功用于测定血清中的汞。我们期望所制备的FNs能在成像、传感和生物分析化学中发挥潜在应用。
