Jørgensen Steffen L, Kudirkiene Egle, Li Lili, Christensen Jens P, Olsen John E, Nolan Lisa, Olsen Rikke H
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, Denmark.
College of Light Industry and Food Sciences, South China University of Technology, Guangdong, Zhongshan Rd, People's Republic of China.
Stand Genomic Sci. 2017 May 10;12:33. doi: 10.1186/s40793-017-0245-3. eCollection 2017.
causing infection outside the gastrointestinal system are referred to as extra-intestinal pathogenic Avian pathogenic is a subgroup of extra-intestinal pathogenic and infections due to avian pathogenic have major impact on poultry production economy and welfare worldwide. An almost defining characteristic of avian pathogenic is the carriage of plasmids, which may encode virulence factors and antibiotic resistance determinates. For the same reason, plasmids of avian pathogenic have been intensively studied. However, genes encoded by the chromosome may also be important for disease manifestation and antimicrobial resistance. For the strain APEC_O2 the plasmids have been sequenced and analyzed in several studies, and APEC_O2 may therefore serve as a reference strain in future studies. Here we describe the chromosomal features of APEC_O2. APEC_O2 is a sequence type ST135, has a chromosome of 4,908,820 bp (plasmid removed), comprising 4672 protein-coding genes, 110 RNA genes, and 156 pseudogenes, with an average G + C content of 50.69%. We identified 82 insertion sequences as well as 4672 protein coding sequences, 12 predicated genomic islands, three prophage-related sequences, and two clustered regularly interspaced short palindromic repeats regions on the chromosome, suggesting the possible occurrence of horizontal gene transfer in this strain. The wildtype strain of APEC_O2 is resistant towards multiple antimicrobials, however, no (complete) antibiotic resistance genes were present on the chromosome, but a number of genes associated with extra-intestinal disease were identified. Together, the information provided here on APEC_O2 will assist in future studies of avian pathogenic strains, in particular regarding strain of APEC_O2, and aid in the general understanding of the pathogenesis of avian pathogenic .
引起胃肠道系统以外感染的被称为肠外致病性大肠杆菌。禽致病性大肠杆菌是肠外致病性大肠杆菌的一个亚群，由禽致病性大肠杆菌引起的感染对全球家禽生产经济和福利有重大影响。禽致病性大肠杆菌几乎一个决定性特征是携带质粒，这些质粒可能编码毒力因子和抗生素抗性决定簇。出于同样的原因，禽致病性大肠杆菌的质粒已得到深入研究。然而，染色体编码的基因对于疾病表现和抗菌抗性也可能很重要。对于APEC_O2菌株，在多项研究中已对其质粒进行了测序和分析，因此APEC_O2可作为未来研究的参考菌株。在此我们描述APEC_O2的染色体特征。APEC_O2是序列型ST135，去除质粒后染色体长度为4,908,820 bp，包含4672个蛋白质编码基因、110个RNA基因和156个假基因，平均G + C含量为50.69%。我们在染色体上鉴定出82个插入序列以及4672个蛋白质编码序列、12个预测的基因组岛、三个前噬菌体相关序列和两个成簇规律间隔短回文重复序列区域，表明该菌株可能发生了水平基因转移。APEC_O2的野生型菌株对多种抗菌药物耐药，然而，染色体上不存在（完整的）抗生素抗性基因，但鉴定出了一些与肠外疾病相关的基因。总之，这里提供的关于APEC_O2的信息将有助于未来对禽致病性大肠杆菌菌株的研究，特别是关于APEC_O2菌株的研究，并有助于对禽致病性大肠杆菌发病机制的总体理解。
