Coe-Pak牙周敷料与Reso-Pac牙周敷料生物效应的体外比较
In Vitro Comparison of Biological Effects of Coe-Pak and Reso-Pac Periodontal Dressings.
作者信息
Kadkhodazadeh Mahdi, Baghani Zahra, Torshabi Maryam, Basirat Bahar
机构信息
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, TehranIran.
Department of Periodontics, School of Dentistry, Sabzevar University of Medical Sciences, SabzevarIran.
出版信息
J Oral Maxillofac Res. 2017 Mar 31;8(1):e3. doi: 10.5037/jomr.2017.8103. eCollection 2017 Jan-Mar.
OBJECTIVES
The purpose of the present study was to compare the cytotoxicity of Reso-Pac and Coe-Pak periodontal dressing.
MATERIAL AND METHODS
According to ISO-10993-12:2012, 1-, 3- and 7-day extracts of the two periodontal dressings were prepared in cell culture medium and exposed to the two cultured cell lines. Cell viability and proliferation at 24 h and 72 h following exposure were evaluated using quantitative MTT assay.
RESULTS
The results showed a significant (P < 0.05) reduction in the viability of cells exposed to the 3- and 7-day Coe-Pak extracts at 24 h and 72 h compared to the control group (no exposure to the extract). Reso-Pac extracts slightly decreased cell viability compared to the control group. Understudy materials showed greater cytotoxicity against human osteoblast-like compared to the human gingival fibroblast cells. No significant (P > 0.05) difference was found in the viability of cells exposed to undiluted (100%) one-day extract and diluted (50%) extract of both understudy materials at 24 h and 72 h after exposure.
CONCLUSIONS
Based on the results, Reso-Pac periodontal dressing has less cytotoxicity than Coe-Pak.
目的
本研究旨在比较Reso - Pac和Coe - Pak牙周敷料的细胞毒性。
材料与方法
根据ISO - 10993 - 12:2012标准，在细胞培养基中制备两种牙周敷料的1天、3天和7天提取物，并将其作用于两种培养的细胞系。使用定量MTT法评估暴露后24小时和72小时的细胞活力和增殖情况。
结果
结果显示，与对照组（未暴露于提取物）相比，暴露于Coe - Pak 3天和7天提取物的细胞在24小时和72小时时活力显著降低（P < 0.05）。与对照组相比，Reso - Pac提取物使细胞活力略有下降。与人类牙龈成纤维细胞相比，受试材料对人成骨样细胞显示出更大的细胞毒性。在暴露后24小时和72小时，暴露于受试材料未稀释（100%）的1天提取物和稀释（50%）提取物的细胞活力未发现显著差异（P > 0.05）。
结论
基于结果，Reso - Pac牙周敷料的细胞毒性低于Coe - Pak。
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