Shu Cindy C, Smith Margaret M, Smith Susan M, Dart Andrew J, Little Christopher B, Melrose James
Raymond Purves Bone and Joint Research Laboratory, Kolling Institute, Northern Sydney Local Health District, St. Leonards, NSW 2065, Australia.
Faculty of Veterinary Science, University Veterinary Teaching Hospital, University of Sydney, Camden, NSW 2050, Australia.
Int J Mol Sci. 2017 May 12;18(5):1049. doi: 10.3390/ijms18051049.
The purpose of this study was to develop a quantitative histopathological scoring scheme to evaluate disc degeneration and regeneration using an ovine annular lesion model of experimental disc degeneration. Toluidine blue and Haematoxylin and Eosin (H&E) staining were used to evaluate cellular morphology: (i) disc structure/lesion morphology; (ii) proteoglycan depletion; (iii) cellular morphology; (iv) blood vessel in-growth; (v) cell influx into lesion; and (vi) cystic degeneration/chondroid metaplasia. Three study groups were examined: 5 × 5 mm lesion; 6 × 20 mm lesion; and 6 × 20 mm lesion plus mesenchymal stem cell (MSC) treatment. Lumbar intervertebral discs (IVDs) were scored under categories (i-vi) to provide a cumulative score, which underwent statistical analysis using STATA software. Focal proteoglycan depletion was associated with 5 × 5 mm annular rim lesions, bifurcations, annular delamellation, concentric and radial annular tears and an early influx of blood vessels and cells around remodeling lesions but the inner lesion did not heal. Similar features in 6 × 20 mm lesions occurred over a 3-6-month post operative period. MSCs induced a strong recovery in discal pathology with a reduction in cumulative histopathology degeneracy score from 15.2 to 2.7 ( = 0.001) over a three-month recovery period but no recovery in carrier injected discs.
本研究的目的是开发一种定量组织病理学评分方案，以使用实验性椎间盘退变的绵羊环形损伤模型来评估椎间盘退变和再生情况。采用甲苯胺蓝和苏木精-伊红（H&E）染色来评估细胞形态：（i）椎间盘结构/损伤形态；（ii）蛋白聚糖耗竭；（iii）细胞形态；（iv）血管长入；（v）细胞流入损伤处；以及（vi）囊性退变/软骨样化生。研究了三个组：5×5毫米损伤组；6×20毫米损伤组；以及6×20毫米损伤加间充质干细胞（MSC）治疗组。对腰椎间盘（IVD）在类别（i - vi）下进行评分以提供累积分数，并使用STATA软件进行统计分析。局灶性蛋白聚糖耗竭与5×5毫米的环形边缘损伤、分叉、环形分层、同心和放射状环形撕裂以及重塑损伤周围血管和细胞的早期流入有关，但内部损伤未愈合。在术后3至6个月期间，6×20毫米损伤中出现了类似特征。间充质干细胞在三个月的恢复期内使椎间盘病理学有了显著恢复，累积组织病理学退变评分从15.2降至2.7（P = 0.001），但载体注射的椎间盘没有恢复。
