Li Xiaohe, Zhang Yunfeng, Song Bing, En He, Gao Shang, Zhang Shaojie, Cai Yongqiang, Li Zhi-Jun, Li Cunbao, Wang Weiping, Wang Xing, Wang Haiyan, Wang Zhiqiang, Zhang Qi, Ma Jierong
Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
Department of CT Center, The Second Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
Med Sci Monit. 2016 Nov 18;22:4426-4430. doi: 10.12659/msm.898062.
BACKGROUND This study provides experimental results on the applicability of bone marrow mesenchymal stem cells (BMSCs) for the repair of intervertebral disc annulus fibrosus in rabbits. MATERIAL AND METHODS Thirty healthy rabbits were randomized into an observation group (n=15) and a control group (n=15). Both groups underwent degeneration of intervertebral disc annulus fibrosus. The observation group was treated with a solution of BMSCs and dexamethasone sodium phosphate, while the control group was treated with dexamethasone sodium phosphate only. RESULTS The two groups were compared for efficacy and pathological conditions after treatment. Both disc height index and level of type II collagen in nucleus pulposus were significantly higher in the observation group than in the control group at 2, 4, 8, and 12 weeks after degeneration (p<0.05 for all comparisons). The percentages of grade 0 and grade 1 were significantly higher in the observation group than in the control group (p<0.05 for both grade 0 and 1 comparisons), while the percentage of grade 4 and grade 5 were significantly lower in the observation group than in the control group (p<0.05 for both grade 4 and 5 comparisons). CONCLUSIONS BMSCs cultured in vitro can effectively repair intervertebral disc annulus fibrosus, which is of positive significance, and thus is clinically recommended.
背景 本研究提供了关于骨髓间充质干细胞（BMSCs）用于修复兔椎间盘纤维环适用性的实验结果。
材料与方法 将30只健康兔随机分为观察组（n = 15）和对照组（n = 15）。两组均进行椎间盘纤维环退变。观察组用BMSCs和地塞米松磷酸钠溶液治疗，而对照组仅用地塞米松磷酸钠治疗。
结果 比较两组治疗后的疗效和病理状况。退变后2、4、8和12周时，观察组的椎间盘高度指数和髓核中II型胶原蛋白水平均显著高于对照组（所有比较p<0.05）。观察组0级和1级的百分比显著高于对照组（0级和1级比较均p<0.05），而观察组4级和5级的百分比显著低于对照组（4级和5级比较均p<0.05）。
结论 体外培养的BMSCs能有效修复椎间盘纤维环，具有积极意义，因此临床推荐使用。
