猪精子附睾成熟过程中碳酸氢盐转运活性的降低。
Decrease in bicarbonate transport activities during epididymal maturation of porcine sperm.
作者信息
Okamura N, Tajima Y, Sugita Y
机构信息
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1280-7. doi: 10.1016/s0006-291x(88)81013-1.
Kinetic studies of [14C]HCO3- and [35S]SO4(2-) transport show the existence of a common transport system for the two anions on the plasma membranes of porcine epididymal sperm. Km value for bicarbonate (8.3mM) is within the range of physiological concentration of bicarbonate. The anion transport activity is dependent on the temperature but independent of co-existence of Na+. Its pH dependency and sensitivity to the several anion transport inhibitors are similar to those observed in erythrocyte. Anion transport activity decreases during sperm maturation in epididymis.
对[14C]HCO3-和[35S]SO4(2-)转运的动力学研究表明，猪附睾精子质膜上存在这两种阴离子的共同转运系统。碳酸氢盐的Km值（8.3mM）在碳酸氢盐生理浓度范围内。阴离子转运活性取决于温度，但与Na+的共存无关。其pH依赖性和对几种阴离子转运抑制剂的敏感性与在红细胞中观察到的相似。在附睾中精子成熟过程中，阴离子转运活性降低。
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