Alghamdi Ahmed N, Mahfouz Mohammad E, Hamdi Fahd A, Al Aboud Daifullah, Al-Laylah Tawfiq Z, Alotaibi Mohammed I, Al-Thomali Khalid W A, Abdel-Moneim Ahmed S
College of Medicine, Taif University, Al-Taif 21944, Saudi Arabia.
College of Medicine, Taif University, Al-Taif 21944, Saudi Arabia; King Faisal Hospital, Al-Taif, Saudi Arabia.
Mol Cell Probes. 2017 Aug;34:59-63. doi: 10.1016/j.mcp.2017.05.002. Epub 2017 May 9.
The 2009 H1N1 pandemic (H1N1pdm09) was associated with a considerable influenza-related morbidity and mortality. Among the complications, Mycobacterial tuberculosis was recorded as a coinfection with influenza in rare cases. The full-length sequences of the viral haemagglutinin and neuraminidase of H1N1pdm09 influenza A virus were analyzed from a recently infected patient. The patient was chronically infected with Mycobacterium tuberculosis. Molecular modelling and in-silico docking of the virus, and other selected strains with the drug oseltamivir were conducted and compared. Sequence analysis of the viral haemagglutinin revealed it to be closely related to the 6B.1 clade, with high identity to the circulating H1N1pdm09 strains, and confirmed that the virus still harbouring high affinity to the α-2,6-sialic acid human receptor. The viral neuraminidase showed high identity to the neuraminidase of the recently circulating strains of the virus with no evidence of the development of oseltamivir-resistant mutants. Regular monitoring of the circulating strains is recommended to screen for a possible emergence of drug-resistant strains.
2009年甲型H1N1流感大流行（H1N1pdm09）导致了相当多与流感相关的发病和死亡情况。在并发症中，分枝杆菌性肺结核在罕见病例中被记录为与流感合并感染。对一名近期感染患者的H1N1pdm09甲型流感病毒的病毒血凝素和神经氨酸酶的全长序列进行了分析。该患者长期感染分枝杆菌性肺结核。对该病毒及其他选定毒株与药物奥司他韦进行了分子建模和计算机模拟对接，并进行了比较。病毒血凝素的序列分析表明，它与6B.1进化枝密切相关，与正在传播的H1N1pdm09毒株具有高度同源性，并证实该病毒仍然对α-2,6-唾液酸人类受体具有高亲和力。病毒神经氨酸酶与该病毒近期传播毒株的神经氨酸酶具有高度同源性，没有证据表明出现了对奥司他韦耐药的突变体。建议定期监测正在传播的毒株，以筛查可能出现的耐药毒株。
