Sage Eva K, Schmid Thomas E, Geinitz Hans, Gehrmann Mathias, Sedelmayr Michael, Duma Marciana N, Combs Stephanie E, Multhoff Gabriele
Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München (TUM), Munich, Germany.
Department of Radiation Sciences (DRS), Institute of Innovate Radiotherapy (iRT), HelmholtzZentrum München, Munich, Germany.
Strahlenther Onkol. 2017 Aug;193(8):648-655. doi: 10.1007/s00066-017-1144-7. Epub 2017 May 12.
Radiotherapy (RT) is an established treatment for patients with primary and recurrent prostate cancer. Herein, the effects of definitive and salvage RT on the composition of lymphocyte subpopulations were investigated in patients with prostate cancer to study potential immune effects.
A total of 33 prostate cancer patients were treated with definitive (n = 10) or salvage RT (n = 23) after biochemical relapse. The absolute number of lymphocytes and the distribution of lymphocyte subpopulations were analyzed by multiparameter flow cytometry before RT, at the end of RT, and in the follow-up period.
Absolute lymphocyte counts decreased significantly after RT in both patient groups and a significant drop was observed in the percentage of B cells directly after RT from 10.1 ± 1.3 to 6.0 ± 0.7% in patients with definitive RT and from 9.2 ± 0.8 to 5.8 ± 0.7% in patients with salvage RT. In contrast, the percentages of T and natural killer (NK) cells remained unaltered directly after RT in both patient groups. However, 1 year after RT, the percentage of CD3 T cells was significantly lower in patients with definitive and salvage RT. The percentage of regulatory T cells was slightly upregulated in primary prostate cancer patients after definitive RT, but not after salvage RT.
Definitive and salvage RT exert similar effects on the composition of lymphocyte subpopulations in prostate cancer patients. Total lymphocyte counts are lower in both patient groups compared to healthy controls and further decreased after RT. B cells are more sensitive to definitive and salvage RT than T and NK cells.
放射治疗（RT）是原发性和复发性前列腺癌患者的既定治疗方法。在此，研究了确定性放疗和挽救性放疗对前列腺癌患者淋巴细胞亚群组成的影响，以探讨潜在的免疫效应。
共有33例前列腺癌患者在生化复发后接受了确定性放疗（n = 10）或挽救性放疗（n = 23）。在放疗前、放疗结束时和随访期间，通过多参数流式细胞术分析淋巴细胞的绝对数量和淋巴细胞亚群的分布。
两组患者放疗后淋巴细胞绝对计数均显著下降，且在放疗结束后即刻观察到B细胞百分比显著下降，确定性放疗患者从10.1±1.3%降至6.0±0.7%，挽救性放疗患者从9.2±0.8%降至5.8±0.7%。相比之下，两组患者放疗结束后即刻T细胞和自然杀伤（NK）细胞百分比保持不变。然而，放疗1年后，确定性放疗和挽救性放疗患者的CD3 T细胞百分比显著降低。原发性前列腺癌患者在接受确定性放疗后调节性T细胞百分比略有上调，但在接受挽救性放疗后未上调。
确定性放疗和挽救性放疗对前列腺癌患者淋巴细胞亚群组成的影响相似。与健康对照相比，两组患者的总淋巴细胞计数均较低，且放疗后进一步降低。B细胞比T细胞和NK细胞对确定性放疗和挽救性放疗更敏感。
