Jing Titao, Yan Lifeng
Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei 230026, Anhui, PR China.
Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei 230026, Anhui, PR China.
Talanta. 2017 Aug 1;170:185-192. doi: 10.1016/j.talanta.2017.03.092. Epub 2017 Mar 31.
A new strategy for pH-responsive near infrared (NIR) cyanine dye, based on the concept of triggered disaggregation-induced emission (DIE), has been developed. Schiff base group was introduced to the molecular scaffold of Cy7, and promoted the aggregation of cyanine in pH7.4 buffer due to the strong π-π interaction. While in an acidic (pH 5.8) environment, the Schiff base group was hydrolyzed by acid and the nanoparticles were disaggregated. The vanishing of the intermolecular aggregation-caused quenching thus induced a fluorescence recovery of 96 folds. Moreover, abundant kinds of Schiff base provide more potential cyanine dyes for pH-responsive ultrahigh NIR fluorescence "Off/On" ratio, which could work as the intelligent bio-imaging probes in near future.
基于触发解聚诱导发射（DIE）概念，开发了一种用于pH响应近红外（NIR）花菁染料的新策略。将席夫碱基团引入Cy7的分子骨架中，由于强烈的π-π相互作用，促进了花菁在pH7.4缓冲液中的聚集。而在酸性（pH 5.8）环境中，席夫碱基团被酸水解，纳米颗粒解聚。分子间聚集导致的猝灭消失，从而诱导荧光恢复96倍。此外，丰富的席夫碱种类为pH响应超高近红外荧光“关/开”比提供了更多潜在的花菁染料，有望在不久的将来用作智能生物成像探针。
