Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China; Department of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.
Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
Talanta. 2017 Aug 1;170:314-321. doi: 10.1016/j.talanta.2017.04.004. Epub 2017 Apr 4.
The sensitive and selective fluorescence probe for hydroxyl radical analysis is of significance because hydroxyl radical plays key roles in many physiological and pathological processes. In this work, a novel organic fluorescence molecular probe OHP for hydroxyl radical is synthesized by a two-step route. The probe employs 4-bora-3a,4a-diaza-s-indacene (difluoroboron dipyrromethene, BODIPY) as the fluorophore and possesses relatively high fluorescence quantum yields (77.14%). Hydroxyl radical can rapidly react with the probe and quench the fluorescence in a good linear relationship (R=0.9967). The limit of detection is determined to be as low as 11nM. In addition, it has been demonstrated that the probe has a good stability against pH and light illumination, low cytotoxicity and high biocompatibility. Cell culture experimental results show that the probe OHP is sensitive and selective for imaging and tracking endogenous hydroxyl radical in live cells.
因为羟基自由基在许多生理和病理过程中起着关键作用，所以用于分析羟基自由基的灵敏和选择性荧光探针具有重要意义。在这项工作中，通过两步法合成了一种新型的有机荧光分子探针 OHP，用于羟基自由基。该探针以 4-硼-3a,4a-二氮杂-s-茚满（二氟化硼二吡咯甲川，BODIPY）为荧光团，具有较高的荧光量子产率（77.14%）。羟基自由基可以迅速与探针反应，并以良好的线性关系猝灭荧光（R=0.9967）。检测限低至 11nM。此外，已经证明该探针具有良好的 pH 值和光照稳定性、低细胞毒性和高生物相容性。细胞培养实验结果表明，探针 OHP 对活细胞内内源性羟基自由基的成像和跟踪具有灵敏和选择性。
