Aboelazm Abeer A, Abd El-Glil Reem R, Elmahdy Mohamed
Department of Microbiology & Immunology,Faculty of Medicine, Benha University, Benha, Egypt.
Department of Chest,Faculty of Medicine, Benha University, Benha, Egypt.
Egypt J Immunol. 2016 Jan;23(1):1-10.
Innate immunity plays an important role in the host defense against Mycobacterium tuberculosis (M. tuberculosis). Toll-like receptor-2 (TLR2) is the main receptor and one of central components in initiation of innate immunity against M. tuberculosis. The study aimed to evaluate TLR2 surface expression on peripheral blood monocytes and study its association with variants in TLR2 597T/C single nucleotide polymorphisms (SNPs) in patients with pulmonary tuberculosis. Fifty pulmonary tuberculosis patients and 50 healthy controls were investigated for TLR2 expression on CD14+ monocytes by flow cytometry and TLR2 597T/C genotyping by Tetra primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). A significant increase (P < 0.001) in TLR 2 expression was detected in tuberculosis patients compared to controls. TLR2 597 TC and CC genotypes were higher in pulmonary tuberculosis patients (OR =2.79, 95% CI =1.02-7.95 and OR =4.26, 95% CI =0.40-214.00) respectively. Genotype TT was associated with reduced risk of being a case of pulmonary tuberculosis. There was no association of TLR2 surface expression on monocytes in pulmonary tuberculosis patients with different TLR2 597T/C genotypes. It is concluded that elevated expression of TLR2 on CD14+ monocytes in pulmonary tuberculosis patients confirms the role of TLR2 in host defense against M. tuberculosis. 597T/C polymorphism of TLR 2 gene may be a risk factor for susceptibility to pulmonary tuberculosis in a sample Egyptian population.
固有免疫在宿主抵御结核分枝杆菌（M. tuberculosis）的防御中发挥着重要作用。Toll样受体2（TLR2）是固有免疫抵御结核分枝杆菌起始过程中的主要受体及核心成分之一。本研究旨在评估外周血单核细胞上TLR2的表面表达，并研究其与肺结核患者TLR2 597T/C单核苷酸多态性（SNP）变异的关联。采用流式细胞术检测50例肺结核患者和50例健康对照者CD14+单核细胞上的TLR2表达，并通过四引物扩增阻滞突变系统-聚合酶链反应（T-ARMS-PCR）对TLR2 597T/C进行基因分型。与对照组相比，肺结核患者的TLR2表达显著增加（P < 0.001）。肺结核患者中TLR2 597 TC和CC基因型较高（OR = 2.79，95% CI = 1.02 - 7.95和OR = 4.26，95% CI = 0.40 - 214.00）。基因型TT与患肺结核风险降低相关。不同TLR2 597T/C基因型的肺结核患者单核细胞上的TLR2表面表达无关联。结论是，肺结核患者CD14+单核细胞上TLR2表达升高证实了TLR2在宿主抵御结核分枝杆菌中的作用。TLR2基因的597T/C多态性可能是埃及样本人群易患肺结核的一个危险因素。
