Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Braz J Infect Dis. 2013 Sep-Oct;17(5):516-20. doi: 10.1016/j.bjid.2012.12.009. Epub 2013 Jul 2.
It is well known that toll-like receptor 2 (TLR2) mediates responses of both innate and adaptive immunity to microbial pathogen, including mycobacteria. Single-nucleotide polymorphisms (SNPs) in the TLR2 gene that impair its function may be associated with the development of pulmonary tuberculosis (PTB). The aim of this study was to evaluate the possible association between TLR2 Arg677Trp and 597T/C polymorphisms and PTB in a sample of Iranian population.
This case-control study was performed on 174 PTB and 177 healthy subjects. Tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) was used to detect the SNPs.
There was no significant difference in the polymorphism of Arg677Trp of the TLR2 gene among PTB and control groups (p>0.05). The results showed that there was a significant difference between case and control groups regarding 597T/C polymorphism (χ(2)=12.21, p=0.002). The TC and CC genotypes were found to be associated with the risk of PTB (OR=2.13, 95% CI=1.25-3.62, p=0.005 and OR=4.88, 95% CI=1.56-15.26, p=0.007, respectively).
Our data suggest that 597T/C polymorphism, but not Arg677Trp polymorphism, of the TLR-2 gene is a risk factor for susceptibility to PTB in a sample of Iranian population.
众所周知，Toll 样受体 2（TLR2）介导先天和适应性免疫对微生物病原体（包括分枝杆菌）的反应。TLR2 基因中功能受损的单核苷酸多态性（SNP）可能与肺结核（PTB）的发展有关。本研究旨在评估 TLR2 Arg677Trp 和 597T/C 多态性与伊朗人群中 PTB 之间的可能关联。
这项病例对照研究在 174 例 PTB 和 177 例健康受试者中进行。四扩增阻滞突变系统-聚合酶链反应（T-ARMS-PCR）用于检测 SNP。
TLR2 基因 Arg677Trp 多态性在 PTB 和对照组之间无显著差异（p>0.05）。结果表明，病例组和对照组之间 597T/C 多态性存在显著差异（χ(2)=12.21，p=0.002）。TC 和 CC 基因型与 PTB 风险相关（OR=2.13，95%CI=1.25-3.62，p=0.005 和 OR=4.88，95%CI=1.56-15.26，p=0.007）。
我们的数据表明，TLR-2 基因的 597T/C 多态性，而不是 Arg677Trp 多态性，是伊朗人群中易患 PTB 的危险因素。
