Badr M, Pelletier G
MRC Group in Molecular Endocrinology, Laval University Medical Center, Ste-Foy, Quebec, Canada.
Mol Cell Endocrinol. 1988 Aug;58(2-3):257-61. doi: 10.1016/0303-7207(88)90162-1.
The cellular and subcellular localization of luteinizing hormone-releasing hormone (LHRH) receptors in rat anterior pituitary has been studied at the light and electron microscopic levels using in vitro autoradiography performed on fixed pituitary sections. The iodinated LHRH agonist [D-Ser(TBU)6,des-Gly-NH10(2)]LHRH ethylamide was used as the ligand. In semithin sections of pituitaries from castrated rats, specific labeling was exclusively localized over the castration cells. At the ultrastructural level, the analysis of silver grain distribution performed in gonadotrophs revealed that the plasma membrane, rough endoplasmic reticulum, and Golgi apparatus were significantly labeled. These findings are consistent with previous reports indicating the presence of specific LHRH binding sites in the plasma membrane. This is also the first demonstration that LHRH receptors are associated with intracytoplasmic organelles. Moreover, this ultrastructural technique should be very useful to localize with precision LHRH receptors in other organs, especially the brain.
利用对固定垂体切片进行的体外放射自显影技术，在光学和电子显微镜水平上研究了促黄体生成激素释放激素（LHRH）受体在大鼠垂体前叶中的细胞和亚细胞定位。碘化LHRH激动剂[D-Ser(TBU)6,des-Gly-NH10(2)]LHRH乙酰胺被用作配体。在去势大鼠垂体的半薄切片中，特异性标记仅定位于去势细胞上。在超微结构水平上，对促性腺激素细胞中银颗粒分布的分析显示，质膜、粗面内质网和高尔基体有明显标记。这些发现与先前报道的质膜中存在特异性LHRH结合位点一致。这也是首次证明LHRH受体与胞质内细胞器相关联。此外，这种超微结构技术对于精确确定其他器官尤其是大脑中的LHRH受体应该非常有用。
