Badr M, Pelletier G
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Ste-Foy, Quebec, Canada.
Neuropeptides. 1988 Jan;11(1):7-11. doi: 10.1016/0143-4179(88)90021-2.
Slide-mounted frozen sections were used for the autoradiographic localization, characterization and quantification of LHRH receptors. The radioligand was the iodinated LHRH agonist [D-Ser(TBU)6, des-Gly-NH2(10)]LHRH ethylamide (LHRH-A). Specificity studies demonstrated that specific labeling was concentrated in the anterior pituitary lobe and that the tracer could be displaced with either LHRH or LHRH-A. Scatchard analysis of saturation curves gave one-site interactions with a high affinity (Kd = 0.48 +/- 0.1 nM) for the iodinated LHRH-A. In order to evaluate the effects of different treatments on the LHRH receptor concentration, the effects of sex steroids on the density of radioautographic reaction were evaluated using an image analyzer. The results obtained were similar to those previously reported with classical binding assays. Thus, this simple technique appears suitable for the study of localization and characterization of LHRH receptors in different experimental conditions.
载玻片上的冷冻切片用于促性腺激素释放激素（LHRH）受体的放射自显影定位、表征和定量分析。放射性配体是碘化LHRH激动剂[D-丝氨酸（叔丁基）6，去甘氨酸-NH2（10）]LHRH乙酰胺（LHRH-A）。特异性研究表明，特异性标记集中在前叶垂体，并且示踪剂可以被LHRH或LHRH-A取代。对饱和曲线的Scatchard分析显示，碘化LHRH-A具有高亲和力（Kd = 0.48 +/- 0.1 nM）的单点相互作用。为了评估不同处理对LHRH受体浓度的影响，使用图像分析仪评估性类固醇对放射自显影反应密度的影响。获得的结果与先前用经典结合测定法报道的结果相似。因此，这种简单的技术似乎适用于研究不同实验条件下LHRH受体的定位和表征。
