Kou Xiaobing, Qi Kaijie, Qiao Xin, Yin Hao, Liu Xing, Zhang Shaoling, Wu Juyou
Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Genomics. 2017 Jul;109(3-4):290-301. doi: 10.1016/j.ygeno.2017.05.003. Epub 2017 May 10.
The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth. However, little is known about CrRLK1L in the wood of the pear fruit tree Pyrus bretchneideri. In this study, 26 CrRLK1L gene members were identified in pear and were grouped into six subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that recent whole genome duplication (WGD) and dispersed gene duplications may contribute to the expansion of the CrRLK1L gene family in pear. Moreover, tissue-specific expression analyses suggested that CrRLK1Ls are involved in the development of various pear tissues. Subsequent qRT-PCR analyses indicated that CrRLK1Ls might play important roles in pollen tube growth. Finally, experiments with antisense oligonucleotides (ASO) demonstrated that PbrCrRLK1L26 have functions in pollen tube elongation and that PbrCrRLK1L3 regulates pollen tube rupture. These results will be useful for elaborating the biological roles of CrRLK1Ls in pear growth and development.
长春花类受体蛋白激酶1样激酶（CrRLK1L）家族参与植物生长过程中的多个进程。然而，对于梨果木本植物砀山梨中的CrRLK1L了解甚少。在本研究中，在梨中鉴定出26个CrRLK1L基因成员，并根据系统发育分析将其分为六个亚家族。进化分析表明，近期的全基因组复制（WGD）和分散的基因复制可能有助于梨中CrRLK1L基因家族的扩展。此外，组织特异性表达分析表明，CrRLK1L参与了梨各种组织的发育。随后的qRT-PCR分析表明，CrRLK1L可能在花粉管生长中发挥重要作用。最后，反义寡核苷酸（ASO）实验证明，PbrCrRLK1L26在花粉管伸长中具有功能，而PbrCrRLK1L3调节花粉管破裂。这些结果将有助于阐明CrRLK1L在梨生长发育中的生物学作用。
