College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China.
School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, China.
Int J Mol Sci. 2023 Feb 5;24(4):3142. doi: 10.3390/ijms24043142.
The receptor-like kinase 1-like (CrRLK1L), which is a vital member of the plant receptor-like kinase family, plays versatile roles in plant growth, development, and stress response. Although the primary screening of tomato CrRLK1Ls has been reported previously, our knowledge of these proteins is still scarce. Using the latest genomic data annotations, a genome-wide re-identification and analysis of the CrRLK1Ls in tomatoes were conducted. In this study, 24 CrRLK1L members were identified in tomatoes and researched further. Subsequent gene structures, protein domains, Western blot analyses, and subcellular localization analyses all confirmed the accuracy of the newly identified SlCrRLK1L members. Phylogenetic analyses showed that the identified SlCrRLK1L proteins had homologs in . Evolutionary analysis indicated that two pairs of the genes had predicted segmental duplication events. Expression profiling analyses demonstrated that the genes were expressed in various tissues, and most of them were up- or down-regulated by bacteria and PAMP treatments. Together, these results will lay the foundation for elaborating the biological roles of SlCrRLK1Ls in tomato growth, development, and stress response.
类受体激酶 1 样蛋白（CrRLK1L）是植物类受体激酶家族的重要成员，在植物生长、发育和应激反应中发挥着多种作用。尽管之前已经对番茄 CrRLK1L 进行了初步筛选，但我们对这些蛋白的了解仍然有限。本研究利用最新的基因组数据注释，对番茄中的 CrRLK1L 进行了全基因组重新鉴定和分析。在这项研究中，我们在番茄中鉴定出了 24 个 CrRLK1L 成员，并对它们进行了进一步研究。随后的基因结构、蛋白结构域、Western blot 分析和亚细胞定位分析都证实了新鉴定的 SlCrRLK1L 成员的准确性。系统发育分析表明，鉴定出的 SlCrRLK1L 蛋白在 中有同源物。进化分析表明，两对 基因发生了预测的片段复制事件。表达谱分析表明，这些基因在各种组织中表达，并且大多数基因在受到细菌和 PAMP 处理时被上调或下调。总之，这些结果将为阐述 SlCrRLK1L 在番茄生长、发育和应激反应中的生物学功能奠定基础。
