Hilsch Malte, Haralampiev Ivan, Müller Peter, Huster Daniel, Scheidt Holger A
Department of Biology, Humboldt University Berlin, Invalidenstraße 43, D-10115 Berlin, Germany.
Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16-18, D-04107 Leipzig, Germany.
Beilstein J Org Chem. 2017 Apr 18;13:720-727. doi: 10.3762/bjoc.13.71. eCollection 2017.
Compared to cholesterol, hydroxycholesterols contain an additional hydroxy group in the alkyl chain and are able to efficiently cross the brain-blood barrier. Therefore, they are responsible for the sterol transfer between brain and circulation. The current study compares the membrane properties of several hydroxycholesterols with those of cholesterol using H NMR spectroscopy, a membrane permeability assay, and fluorescence microscopy experiments. It is shown that hydroxycholesterols do not exert the unique impact on membrane properties characteristic for cholesterol with regard to the influence on lipid chain order, membrane permeability and formation of lateral domains.
与胆固醇相比，羟基胆固醇在烷基链中含有一个额外的羟基，并且能够有效地穿过血脑屏障。因此，它们负责大脑与循环系统之间的甾醇转运。本研究使用核磁共振氢谱、膜通透性测定和荧光显微镜实验，比较了几种羟基胆固醇与胆固醇的膜特性。结果表明，在对脂质链有序性、膜通透性和侧向结构域形成的影响方面，羟基胆固醇对膜特性没有产生胆固醇所特有的独特影响。
