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25-羟胆固醇对膜结构和力学性能的影响。

25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties.

机构信息

Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal.

Centro de Investigación en Biofísica Aplicada y Alimentos (CIBAAL), Universidad Nacional de Santiago Del Estero-CONICET, Santiago del Estero 4206, Argentina.

出版信息

Int J Mol Sci. 2021 Mar 4;22(5):2574. doi: 10.3390/ijms22052574.

DOI:10.3390/ijms22052574
PMID:33806504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7961727/
Abstract

Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses' entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25-hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25-hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)-enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.

摘要

胆固醇负责调节质膜的柔韧性，并参与生理和病理生理反应。胆固醇稳态受氧化固醇（如 25-羟胆固醇）调节。研究表明，质膜水平的 25-羟胆固醇会干扰几种病毒进入靶细胞。我们使用原子力显微镜来评估 25-羟胆固醇对具有受控脂质组成的不同性质的支撑脂质双层的影响。具体来说，我们表明，25-羟胆固醇抑制胆固醇的脂质浓缩作用，使双层更具柔韧性。本研究表明，25-羟胆固醇包含在质膜中或其胆固醇含量的一部分转化为 25-羟胆固醇，会导致富含神经鞘磷脂（SM）的域的形态改变，并促进脂质堆积不均匀性。这些变化最终导致膜硬度变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/e2e43bf47f90/ijms-22-02574-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/9bf53dc80e97/ijms-22-02574-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/151fe13c16aa/ijms-22-02574-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/4d3f0e1f6702/ijms-22-02574-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/e2e43bf47f90/ijms-22-02574-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/9bf53dc80e97/ijms-22-02574-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/3b6264be4812/ijms-22-02574-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/ae24f57f6fa0/ijms-22-02574-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/151fe13c16aa/ijms-22-02574-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/4d3f0e1f6702/ijms-22-02574-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6412/7961727/e2e43bf47f90/ijms-22-02574-g006.jpg

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