Oono Masahiro, Yamaguchi Keisuke, Rasyid Amirul, Takano Atsushi, Tanaka Masato, Futai Nobuyuki
Department of Mechanical Engineering, Graduate School of Engineering and Science, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Department of Mechanical Engineering, College of Engineering, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Biomicrofluidics. 2017 May 3;11(3):034103. doi: 10.1063/1.4983148. eCollection 2017 May.
Various microfluidic features, such as traps, have been used to manipulate flows, cells, and other particles within microfluidic systems. However, these features often become undesirable in subsequent steps requiring different fluidic configurations. To meet the changing needs of various microfluidic configurations, we developed a reconfigurable microfluidic channel with movable sidewalls using mechanically discretized sidewalls of laterally aligned rectangular pins. The user can deform the channel sidewall at any time after fabrication by sliding the pins. We confirmed that the flow resistance of the straight microchannel could be reversibly adjusted in the range of 10-10Pa s/l by manually displacing one of the pins comprising the microchannel sidewall. The reconfigurable microchannel also made it possible to manipulate flows and cells by creating a segmented patterned culture of COS-7 cells and a coculture of human umbilical vein endothelial cells (HUVECs) and human lung fibroblasts (hLFs) inside the microchannel. The reconfigurable microfluidic device successfully maintained a culture of COS-7 cells in a log phase throughout the entire period of 216 h. Furthermore, we performed a migration assay of cocultured HUVEC and hLF spheroids within one microchannel and observed their migration toward each other.
各种微流体特征，如阱，已被用于在微流体系统中操纵流体、细胞和其他颗粒。然而，在需要不同流体配置的后续步骤中，这些特征往往变得不理想。为了满足各种微流体配置不断变化的需求，我们开发了一种具有可移动侧壁的可重构微流体通道，该通道使用横向对齐的矩形销的机械离散侧壁。用户可以在制造后通过滑动销随时使通道侧壁变形。我们证实，通过手动移动构成微通道侧壁的其中一个销，直微通道的流动阻力可以在10-10Pa·s/l的范围内可逆地调节。这种可重构微通道还通过在微通道内创建COS-7细胞的分段图案化培养以及人脐静脉内皮细胞（HUVEC）和人肺成纤维细胞（hLF）的共培养，实现了对流体和细胞的操纵。这种可重构微流体装置在整个216小时的时间段内成功地将COS-7细胞培养维持在对数期。此外，我们在一个微通道内对共培养的HUVEC和hLF球体进行了迁移试验，并观察到它们相互迁移。
