Fowler C J, Thorell G, Sundström E, Archer T
Research and Development Laboratories, Astra Alab AB, Södertälje, Sweden.
J Neural Transm. 1988;73(3):205-15. doi: 10.1007/BF01250137.
Norepinephrine (NE)-stimulated inositol phospholipid hydrolysis ("PI breakdown") in rat cerebral cortical miniprisms was used as a measure of alpha 1-adrenoceptor function following serotonin and/or NE depletion. The use of ascorbic acid to prevent autooxidation of the NE during the PI breakdown assay was found to be warranted. Treatment of rats with 5,7-dihydroxytryptamine and DSP4 produced selective depletions of serotonin (79-95%) and NE (69-85%), respectively, in cortical and hippocampal brain regions. The degree of cortical NE-stimulated PI breakdown in the lesioned animals was not significantly different from that in the control animals, suggesting that under the conditions used, serotonin and NE depletion do not lead to a changed sensitivity of alpha 1-adrenoceptors coupled to PI breakdown in the rat cortex.
在5-羟色胺和/或去甲肾上腺素耗竭后，用去甲肾上腺素（NE）刺激大鼠大脑皮质微小组织中的肌醇磷脂水解（“磷脂酰肌醇分解”）作为α1-肾上腺素能受体功能的一项指标。发现在磷脂酰肌醇分解测定过程中使用抗坏血酸来防止NE的自动氧化是必要的。用5,7-二羟基色胺和DSP4处理大鼠分别在皮质和海马脑区选择性地耗竭了5-羟色胺（79-95%）和去甲肾上腺素（69-85%）。损伤动物中皮质NE刺激的磷脂酰肌醇分解程度与对照动物相比无显著差异，这表明在所使用的条件下，5-羟色胺和去甲肾上腺素的耗竭不会导致与大鼠皮质中磷脂酰肌醇分解偶联的α1-肾上腺素能受体敏感性改变。
