Li Haiming, Chai Shoudong, Dai Longsheng, Gu Chengxiong
Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China.
Biomed Res Int. 2017;2017:7473437. doi: 10.1155/2017/7473437. Epub 2017 Apr 19.
. The aim of this study was to test the effects of collagen external scaffold (CES) in intimal hyperplasia of vein grafts and explore its underlying mechanisms. . Thirty-six New Zealand white rabbits were randomized into no-graft group, graft group, and CES group. The rabbit arteriovenous graft model was established. In CES group, the vein graft was wrapped around with CES. The hemodynamic parameters of vein grafts were measured intraoperatively and 4 weeks after operation by ultrasonic examination. Histological characteristics of vein grafts were also evaluated 4 weeks later. The mRNA and protein levels of proliferating cell nuclear antigen (PCNA), active cleaved-caspase-3 (ClvCasp-3), and smooth muscle 22 alpha (SM22) were measured 4 weeks later by quantitative real-time PCR and western blot. . CES significantly improved the hemodynamic stability of vein grafts, with higher blood velocity and blood flow. Similarly, CES also markedly mitigated intimal hyperplasia and inhibited dilatation of vein grafts. In CES group, the upexpression of PCNA and ClvCasp-3 and the downexpression of SM22 were inhibited. . CES exerts beneficial effects in mitigating intimal hyperplasia and improving remodeling of autogenous vein grafts, which may be associated with reducing the proliferation and apoptosis and preserving the phenotype of VSMCs.
本研究旨在测试胶原蛋白外部支架（CES）对静脉移植物内膜增生的影响，并探讨其潜在机制。将36只新西兰白兔随机分为无移植组、移植组和CES组。建立兔动静脉移植模型。在CES组中，用CES包裹静脉移植物。术中及术后4周通过超声检查测量静脉移植物的血流动力学参数。术后4周也评估静脉移植物的组织学特征。术后4周通过定量实时PCR和蛋白质印迹法测量增殖细胞核抗原（PCNA）、活性裂解型半胱天冬酶-3（ClvCasp-3）和平滑肌22α（SM22）的mRNA和蛋白质水平。CES显著改善了静脉移植物的血流动力学稳定性，具有更高的血流速度和血流量。同样，CES也显著减轻了内膜增生并抑制了静脉移植物的扩张。在CES组中，PCNA和ClvCasp-3的上调以及SM22的下调受到抑制。CES在减轻内膜增生和改善自体静脉移植物重塑方面发挥有益作用，这可能与减少增殖和凋亡以及保留血管平滑肌细胞表型有关。
