Scholz Fiona M, Burrows Amanda K, Griffin Craig E, Muse Russell
Animal Dermatology Clinic, Perth Veterinary Specialists, 305 Selby Street North, Osborne Park, WA, 6017, Australia.
Animal Dermatology Clinic, Murdoch University Veterinary Hospital, 90 South Street, Murdoch, WA, 6150, Australia.
Vet Dermatol. 2017 Aug;28(4):351-e78. doi: 10.1111/vde.12320. Epub 2017 May 14.
Currently the same allergen concentrations for canine intradermal testing (IDT) are recommended for feline IDT. Feline skin reactions are subtle and more difficult to read than canine reactions. This difference may be due to suboptimal allergen concentrations used for IDT in cats.
HYPOTHESIS/OBJECTIVES: To determine the irritant threshold concentration (ITC) of 16 pollen allergens using serial dilutions of allergen and intravenous fluorescein. The hypothesis tested was that feline IDT currently is performed at suboptimal allergen concentrations for pollens.
Twenty privately owned healthy clinically nonallergic cats.
IDT was performed in duplicate using 16 pollen allergens (weeds, grasses and trees) at a dilution of 8000 PNU/mL. Two blinded investigators graded the test reactions independently using subjective and objective criteria. Intravenous fluorescein was then administered and the test reactions were re-evaluated. IDT was repeated for any allergen that was positive, using serial dilutions of allergen at a concentration of 6000 and 4000 PNU/mL.
The ITC for 2 of 16 of the allergens was determined. The ITC of Cynodon dactylon (Bermuda grass) and Schinus spp. (Peppercorn) was determined to be between 6000 and 8000 PNU/mL. The ITC of all other allergens tested in this study was >8000 PNU/mL.
This study confirms that suboptimal allergen concentrations currently are used for feline IDT as the ITC is >8000 PNU/mL for 14 of 16 of the grass, weed and tree pollens evaluated. The ITC of Cynodon dactylon and Schinus spp. was determined to be between 6000 and 8000 PNU/mL.
目前犬类皮内试验（IDT）所推荐的变应原浓度同样用于猫类IDT。猫的皮肤反应较为细微，比犬类反应更难判读。这种差异可能是由于猫类IDT所使用的变应原浓度并非最适宜浓度。
假设/目的：通过变应原系列稀释液和静脉注射荧光素，测定16种花粉变应原的刺激阈浓度（ITC）。所检验的假设为，目前猫类IDT所使用的花粉变应原浓度并非最适宜浓度。
20只 privately owned健康的临床无过敏反应的猫。
使用16种花粉变应原（杂草、禾本科植物和树木），以8000 PNU/mL的稀释度进行重复IDT。两名盲法研究者分别使用主观和客观标准对试验反应进行分级。随后静脉注射荧光素，并重新评估试验反应。对于任何呈阳性的变应原，使用浓度为6000和4000 PNU/mL的变应原系列稀释液重复进行IDT。
确定了16种变应原中2种变应原的ITC。狗牙根（百慕大草）和肖乳香属（胡椒）的ITC测定值在6000至8000 PNU/mL之间。本研究中所检测的所有其他变应原的ITC均>8000 PNU/mL。
本研究证实，目前猫类IDT所使用的变应原浓度并非最适宜浓度，因为在所评估的16种禾本科植物、杂草和树木花粉中，有14种的ITC>8000 PNU/mL。狗牙根和肖乳香属的ITC测定值在6000至8000 PNU/mL之间。
