Hensel Patrick, Austel Michaela, Medleau Linda, Zhao Ying, Vidyashankar Anand
Department of Small Animal Medicine, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA.
Vet Dermatol. 2004 Oct;15(5):304-8. doi: 10.1111/j.1365-3164.2004.00400.x.
The purpose of this study was to determine the optimal histamine concentration and allergen threshold concentrations for canine intradermal testing. Thirty healthy dogs were tested using two different concentrations of histamine and four different concentrations of each allergen. The optimal histamine concentration was determined to be 1:10 000 w/v. The threshold concentration was at least 1750 PNU/mL for all tested grasses, weeds, trees, moulds and insects, except for fleas which was as least 1:500 w/v. For Dermatophagoides pteronyssinus, the optimal threshold concentration was 250 PNU/mL, whereas for Dermatophagoides farinae and Tyrophagus putrescentiae, it was 100 PNU/mL. Threshold concentration for all epidermals except human dander was at least 1250 PNU/mL. The optimal threshold concentration for human dander was 300 PNU/mL. Our results suggest that the currently used 1:100 000 w/v concentration of histamine and the 1000 PNU/mL concentration for most grasses, weeds, trees, moulds, epidermals and insects may not be appropriate for canine intradermal testing.
本研究的目的是确定犬皮内试验的最佳组胺浓度和变应原阈值浓度。使用两种不同浓度的组胺和每种变应原的四种不同浓度对30只健康犬进行测试。确定最佳组胺浓度为1:10 000 w/v。除跳蚤的阈值浓度至少为1:500 w/v外，所有测试的草、杂草、树、霉菌和昆虫的阈值浓度至少为1750 PNU/mL。对于屋尘螨，最佳阈值浓度为250 PNU/mL，而对于粉尘螨和腐食酪螨，最佳阈值浓度为100 PNU/mL。除人皮屑外，所有表皮的阈值浓度至少为1250 PNU/mL。人皮屑的最佳阈值浓度为300 PNU/mL。我们的结果表明，目前用于犬皮内试验的1:100 000 w/v组胺浓度以及大多数草、杂草、树、霉菌、表皮和昆虫的1000 PNU/mL浓度可能不合适。
