Ahmed Tanzeel, Goel Vasu, Banerjee B D
a Department of Biotechnology, School of Engineering and Technology , IFTM University, Lodhipur Rajput , Moradabad , India.
b Enivironmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry , University College of Medical Sciences and GTB Hospital, University of Delhi , Delhi , India.
Drug Chem Toxicol. 2018 Apr;41(2):128-134. doi: 10.1080/01480545.2017.1321010. Epub 2017 May 15.
The present study enumerates the attenuating effects of curcumin and α-tocopherol against propoxur induced oxidative DNA damage in human peripheral blood mononuclear cells (PBMC). Cultured cells were isolated from peripheral blood of healthy volunteers, and were exposed to varying concentrations of propoxur (0-21 μg/ml) for 6, 12, and 24 h, and in combination with curcumin (9.2 μg/ml) or α-tocopherol (4.3 μg/ml) or both. Cytotoxic effect of propoxur was examined by MTT assay. The role of oxidative stress beneath the cytotoxicity of propoxur was evaluated by the measurement of reduced glutathione (GSH), malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels in cell lysate. A concentration-dependent cell death, depletion of GSH, an increase in the level of both MDA and 8-OH-dG were observed. Co-treatment with curcumin or α-tocopherol significantly attenuates depleted GSH, decrease in MDA and 8-OH-dG levels in propoxur exposed cells (p < 0.05). The results of the present study provide experimental evidence of involvement of oxidative stress in propoxur-mediated genotoxicity in human PBMC and highlight the antioxidant role of curcumin and α-tocopherol following propoxur exposure.
本研究列举了姜黄素和α-生育酚对人类外周血单个核细胞（PBMC）中残杀威诱导的氧化性DNA损伤的减轻作用。从健康志愿者的外周血中分离培养细胞，并将其暴露于不同浓度的残杀威（0 - 21μg/ml）中6、12和24小时，并与姜黄素（9.2μg/ml）或α-生育酚（4.3μg/ml）或两者联合使用。通过MTT法检测残杀威的细胞毒性作用。通过测量细胞裂解液中还原型谷胱甘肽（GSH）、丙二醛（MDA）和8-羟基-2'-脱氧鸟苷（8-OH-dG）的水平，评估氧化应激在残杀威细胞毒性中的作用。观察到浓度依赖性的细胞死亡、GSH的消耗、MDA和8-OH-dG水平的升高。与姜黄素或α-生育酚联合处理可显著减轻残杀威暴露细胞中GSH的消耗、MDA和8-OH-dG水平的降低（p < 0.05）。本研究结果为氧化应激参与残杀威介导的人类PBMC基因毒性提供了实验证据，并突出了姜黄素和α-生育酚在残杀威暴露后的抗氧化作用。
