Fernández Miguel, Fuertes Miguel, Elguezabal Natalia, Castaño Pablo, Royo Marcos, Ferreras M Carmen, Benavides Julio, Pérez Valentín
Dpto de Sanidad Animal, Instituto de Ganadería de Montaña (CSIC-Universidad de León), Facultad de Veterinaria, Campus de Vegazana s/n, 24071, León, Spain.
Dpto de Sanidad Animal, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, 48160 Derio, Bizkaia, Spain.
Comp Immunol Microbiol Infect Dis. 2017 Apr;51:1-8. doi: 10.1016/j.cimid.2017.01.002. Epub 2017 Feb 7.
Animals infected with Mycobacterium avium subsp. paratuberculosis (Map) show a variety of lesions, from focal forms, seen in subclinical stages to diffuse lesions in clinical cases. The purpose of this study was to evaluate the local expression of IFN-γ by immunohistochemistry in relation with the type of lesion in naturally Map-infected cows. The number of immunolabelled cells, -the majority morphologically consistent with lymphocytes-, was higher in focal and diffuse paucibacillary forms than in diffuse multibacillary lesions, where they appeared closely related to epithelioid cells. Diffuse multibacillary lesions had the lowest numbers, but higher than controls, and positive cells were intermingled among the macrophages. The peripheral IFN-γ production was higher in all Map infected cows and a positive correlation was found with the number of immunolabelled cells in the intestine. The findings of this study show that IFN-γ would play a role in the development of the different types of lesions in paratuberculosis, and also points out the importance of adequate sampling of lymphoid tissue containing samples when studying the local immune response in which IFN-γ expression may be involved, especially in cases where focal lesions are present.
感染副结核分枝杆菌鸟亚种（Map）的动物会出现多种病变，从亚临床阶段可见的局灶性病变到临床病例中的弥漫性病变。本研究的目的是通过免疫组织化学评估自然感染Map的奶牛中IFN-γ的局部表达与病变类型的关系。免疫标记细胞的数量——大多数在形态上与淋巴细胞一致——在局灶性和弥漫性少菌型病变中高于弥漫性多菌型病变，在弥漫性多菌型病变中它们似乎与上皮样细胞密切相关。弥漫性多菌型病变中的数量最少，但高于对照组，阳性细胞夹杂在巨噬细胞之间。所有感染Map的奶牛外周血IFN-γ产量均较高，且与肠道中免疫标记细胞的数量呈正相关。本研究结果表明，IFN-γ在副结核不同类型病变的发展中起作用，并且还指出在研究可能涉及IFN-γ表达的局部免疫反应时，对含淋巴组织样本进行充分采样的重要性，特别是在存在局灶性病变的情况下。
