CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Carbohydr Polym. 2017 Aug 1;169:357-365. doi: 10.1016/j.carbpol.2017.04.030. Epub 2017 Apr 17.
The present study reports the development of non-cytotoxic and pH-sensitive nanostructured membranes consisting of a polymer with amino acid pending moieties and bacterial nanocellulose (BC). The nanocomposites were prepared through a simple methodology under green reaction conditions. The obtained materials display good thermal stability (up to 200°C), viscoelastic (storage modulus>700MPa) and mechanical (Young's modulus=3.5-4.9GPa) properties, together with high water uptake capacity. The results of the in vitro MTT assay showed that the nanocomposites are non-cytotoxic to HaCaT cells for 72h. The in vitro release profile of diclofenac sodium salt (DCF) from the nanocomposites into simulated body fluids at different pH values demonstrates the pH-responsive behaviour of these materials. Besides, DCF is mainly retained in the nanocomposites at pH 2.1 and released at pH 7.4, revealing their potential for the controlled release of DCF in dermal as well as in oral drug delivery applications.
本研究报告了由具有氨基酸悬垂基团的聚合物和细菌纳米纤维素（BC）组成的非细胞毒性和 pH 敏感的纳米结构膜的开发。通过在绿色反应条件下的简单方法制备了纳米复合材料。所得到的材料显示出良好的热稳定性（高达 200°C）、粘弹性（储能模量>700MPa）和机械性能（杨氏模量=3.5-4.9GPa），以及高吸水性。体外 MTT 测定结果表明，纳米复合材料在 72 小时内对 HaCaT 细胞无细胞毒性。在不同 pH 值的模拟体液中，双氯芬酸钠盐（DCF）从纳米复合材料中的体外释放曲线表明了这些材料的 pH 响应行为。此外，DCF 在 pH 2.1 主要保留在纳米复合材料中，在 pH 7.4 释放，表明它们在皮肤和口服药物递送应用中 DCF 控制释放的潜力。
