Sakihama K, Eizuru Y, Minamishima Y
Department of Microbiology, Miyazaki Medical College.
Microbiol Immunol. 1988;32(9):933-47. doi: 10.1111/j.1348-0421.1988.tb01455.x.
The interaction between herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) and two neural cell lines, mouse neuroblastoma (N1E-115) and rat glioma (C6-BU-1), was investigated. N1E-115 cells were permissive to both types of HSV. In C6-BU-1 cells, on the other hand, all the HSV-1 strains tested so far showed persistent infection, and the infectious virus of HSV-2 strains disappeared spontaneously. The HSV-2-infected C6-BU-1 cells were positive for HSV-2-specific DNA sequences, virus-specific RNA, HSV-2-specific antigens and thymidine kinase activity, when no infectious virus was detected. The HSV-2 was reactivated from those C6-BU-1 cells by superinfection with murine cytomegalovirus (MCMV), but not with UV-irradiated MCMV or human cytomegalovirus. The reactivated HSV-2 was identical to the parental virus, when examined by restriction endonuclease cleavage analysis.
研究了1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）与两种神经细胞系，即小鼠神经母细胞瘤（N1E-115）和大鼠胶质瘤（C6-BU-1）之间的相互作用。N1E-115细胞对两种类型的HSV均具有易感性。另一方面，在C6-BU-1细胞中，迄今为止测试的所有HSV-1毒株均表现出持续感染，而HSV-2毒株的感染性病毒则自发消失。在未检测到感染性病毒时，感染HSV-2的C6-BU-1细胞对HSV-2特异性DNA序列、病毒特异性RNA、HSV-2特异性抗原和胸苷激酶活性呈阳性。通过用鼠巨细胞病毒（MCMV）重复感染，而非用紫外线照射的MCMV或人巨细胞病毒重复感染，可使HSV-2从那些C6-BU-1细胞中重新激活。通过限制性内切酶切割分析检测时，重新激活的HSV-2与亲代病毒相同。
