Barbieri N, Herrera M, Salva S, Villena J, Alvarez S
1 Laboratorio de Inmunobiotecnología, Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, Tucumán (CP 4000), Argentina.
2 Departamento de Ciencias Básicas y Tecnológicas, Universidad Nacional de Chilecito, 9 de Julio 22, Chilecito (F5360CKB), La Rioja, Argentina.
Benef Microbes. 2017 May 30;8(3):393-405. doi: 10.3920/BM2016.0152. Epub 2017 May 15.
Immunobiotic lactic acid bacteria have become an interesting alternative for the prevention of respiratory infections. Previously, we demonstrated that the nasal administration of Lactobacillus rhamnosus CRL1505, during repletion of malnourished mice, resulted in diminished susceptibility to the challenge with the respiratory pathogen Streptococcus pneumoniae. Considering the known alterations induced by malnutrition on T lymphocytes and the importance of this cell population on the protection against respiratory pathogens, we aimed to study the effect of L. rhamnosus CRL1505 nasal administration on the recovery of T cell-mediated defences against pneumococcal infection in malnourished mice under nutritional recovery. Malnourished mice received a balanced conventional diet (BCD) for seven days or BCD for seven days with nasal L. rhamnosus CRL1505 supplementation during last two days of the treatment. After the treatments mice were infected with S. pneumoniae. Flow cytometry studies were carried out in bone marrow, thymus, spleen and lung to study T cells, and Th/Th cytokine profiles were determined in broncho-alveolar lavages and serum. The administration of CRL1505 strain to malnourished mice under recovery reduced quantitative and qualitative alterations of CD4 T cells in the bone marrow, thymus, spleen and lung induced by malnutrition. In addition, CRL1505 treatment augmented Th-cytokines (interleukin 10 and 4) in respiratory and systemic compartments after pneumococcal infection. These results show that modulation of CD4 T lymphocytes induced by L. rhamnosus CRL1505 has an important role in the beneficial effect induced by this strain on the recovery of malnourished mice. These data also indicate that nasally administered L. rhamnosus CRL1505 may represent a non-invasive alternative to modulate and improve the T cell-mediated immunity against respiratory pathogens in immunocompromised malnourished hosts.
免疫益生菌乳酸菌已成为预防呼吸道感染的一个有趣的替代方法。此前，我们证明，在营养不良小鼠营养补充期间经鼻给予鼠李糖乳杆菌CRL1505，可降低其对呼吸道病原体肺炎链球菌攻击的易感性。鉴于已知营养不良会引起T淋巴细胞的改变，以及该细胞群体在抵御呼吸道病原体方面的重要性，我们旨在研究在营养恢复过程中，经鼻给予鼠李糖乳杆菌CRL1505对营养不良小鼠T细胞介导的抗肺炎球菌感染防御功能恢复的影响。营养不良小鼠接受7天的均衡常规饮食（BCD），或在治疗的最后两天接受7天的BCD并经鼻补充鼠李糖乳杆菌CRL1505。治疗后，小鼠感染肺炎链球菌。对骨髓、胸腺、脾脏和肺进行流式细胞术研究以分析T细胞，并在支气管肺泡灌洗液和血清中测定Th/Th细胞因子谱。在营养恢复过程中，给营养不良小鼠施用CRL1505菌株可减少营养不良引起的骨髓、胸腺、脾脏和肺中CD4 T细胞的数量和质量改变。此外，CRL1505治疗可增加肺炎球菌感染后呼吸道和全身各部位的Th细胞因子（白细胞介素10和4）。这些结果表明，鼠李糖乳杆菌CRL1505诱导的CD4 T淋巴细胞调节在该菌株对营养不良小鼠恢复的有益作用中起重要作用。这些数据还表明，经鼻给予鼠李糖乳杆菌CRL1505可能是一种非侵入性的替代方法，可调节和改善免疫功能低下的营养不良宿主中T细胞介导的针对呼吸道病原体的免疫力。
