Yang G, Jiang Y, Tong P, Li C, Yang W, Hu J, Ye L, Gu W, Shi C, Shan B, Wang C
1 College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, Jilin Province 130118, China P.R.
2 Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China P.R.
Benef Microbes. 2017 May 30;8(3):379-391. doi: 10.3920/BM2016.0116. Epub 2017 May 15.
FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19 B cells and CD11cDC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.
FaeG是K88菌毛的主要亚基。这些细胞表面附着物被认为是产肠毒素大肠杆菌（ETEC）的主要毒力因子，ETEC可导致仔猪腹泻。使用树突状细胞靶向肽（DCpep）已被证明是增强疫苗免疫的有效方法。植物乳杆菌因其有益作用和安全性，是口服疫苗的有吸引力的候选者。在本研究中，使用植物乳杆菌递送FaeG-DCpep融合抗原，并评估小鼠的免疫反应。FaeG-DCpep的合成显著增加了重组植物乳杆菌（RLP）对IPEC-J2细胞表面的粘附，导致在粘附试验中植物乳杆菌和ETEC之间的直接竞争。在用RLP免疫的BALB/c小鼠中观察到体重增加、血清免疫球蛋白G和肠道免疫球蛋白A水平显著更高。此外，与未免疫小鼠相比，脾脏和淋巴结中CD19 B细胞和CD11c DC细胞的数量以及几种细胞因子的表达水平显著增加。苏木精-伊红染色显示，口服RLP还减轻了ETEC攻击的症状，表明RLP可能是一种有效的候选疫苗。
