Markwardt F, Albitz R, Franke T, Nilius B
Julius Bernstein Institute of Physiology, Martin Luther University, Halle (Saale), German Democratic Republic.
Pflugers Arch. 1988 Oct;412(6):668-70. doi: 10.1007/BF00583771.
Effects of the proteolytic enzyme thrombin in the modulation of cardiac Ca-channel currents were examined in single ventricular cells from frog myocardium, using the whole-cell voltage clamp technique (Hamill et al. 1981). Application of 3.8.10(-9) M thrombin to the bath increased the peak of the Ca-channel current by 84 +/- 35% (8 cells). Hirudin (31.10(-9) M), a specific thrombin inhibitor, blocked the thrombin-induced increase of this current. The increase in the current can be made responsible for the measured positive inotropic effects of thrombin as well as for the shift of the plateau voltage of the action potentials towards more positive values.
采用全细胞膜片钳技术（Hamill等人，1981年），在青蛙心肌单个心室细胞中研究了蛋白水解酶凝血酶对心脏钙通道电流的调节作用。向浴槽中加入3.8×10⁻⁹ M凝血酶可使钙通道电流峰值增加84±35%（8个细胞）。特异性凝血酶抑制剂水蛭素（31×10⁻⁹ M）可阻断凝血酶诱导的该电流增加。电流增加可解释凝血酶所测得的正性肌力作用以及动作电位平台期电压向更正的值的偏移。
