凝血酶可增加离体蛙心室细胞中的钙通道电流。
Ca-channel currents in isolated frog ventricular cells are increased by thrombin.
作者信息
Albitz R, Markwardt F, Franke T, Nilius B
机构信息
Julius Bernstein Institute of Physiology, Martin Luther University, Halle, GDR.
出版信息
Biomed Biochim Acta. 1989;48(5-6):S350-3.
Effects of the proteolytic enzyme thrombin in the modulation of cardiac Ca-channel currents were examined in single ventricular cells from frog myocardium, using the whole-cell voltage clamp technique (1). Application of 3.8 . 10(-9) M thrombin to the bath increased the peak of the Ca-channel current by 84 +/- 35% (8 cells). Hirudin (31.10(-9)M), a specific thrombin inhibitor, blocked the thrombin-induced increase of this current. The increase in the current can be made responsible for the measured positive inotropic effects on frog heart of thrombin.
采用全细胞膜片钳技术，研究了蛋白水解酶凝血酶对青蛙心肌单个心室细胞中心脏钙通道电流的调节作用(1)。向浴槽中加入3.8×10(-9)M凝血酶，可使钙通道电流峰值增加84±35%(8个细胞)。特异性凝血酶抑制剂水蛭素(31×10(-9)M)可阻断凝血酶诱导的该电流增加。电流增加可能是凝血酶对青蛙心脏产生正性肌力作用的原因。
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